05: Import Multidimensional Image
In this example, I try to import a 3D image object that has 240 image files, 6 columns and 8 rows in 5 different colors
Click on Import tab
Select type of object as image
Select Dimension as 3D (eg. image timelapse)
Type in an Object name (eg. myImage)
Click on Choose files button
Click on Load button to choose multiple images. Press Shift to choose multiple images.
Click Open
Get back to Choose files window and the image you select will show up under the Load button
If you only want to filter these images, you can check the corresponding checkboxes and click on re-filter.
Click Ok
You will get back to the workplace window
Again, I want to import a 3D image object that has 240 image files, 6 columns and 8 rows in 5 different colors.
Under “File dealing order”
You need to indicate how to assign dimension values to each file in the list.
Type in a name for each dimension in the order such that the first dimension listed in the inner-most loop of a nested for-loop of dimensions used for assigning dimension indices and so on.
In my case, I have my images in such order: the first 5 files are images in different color of same coordinate (eg. x0_y0) while images 6-10 are images in different color of next coordinate (eg. x0_y1)
Since color is the inner-most loop, I enter first dimension as color and enter the size of it as 5.
Since y coordinate is the outer loop, I enter second dimension as imRow – image row and enter the size of it as 8.
Since x coordinate is the outer-most loop, I enter third dimension as imCol – image column and enter the size of it as 6.
"Array Row" and "Array Col" are reserved dimension names for the Dataset rows and columns.
Recall that when you create a Dataset you can indicate an array of entries (those square wells on the left hand side). Then if you are importing images into multiple entries, pay attention to where in the list these special dimension names fall. If you are importing into a single Entry, use the default.
Click on “Deal files!” button in the bottom right
The name of the image that you import will show up under Cell 0.0
Click on Import button
Now you can see a new Image object called myImage on the left which is the image you have just imported.
To open the image, double click on the icon in front of the image name.
To open the image, double click on the icon in front of the image name.
Drag the Max and Min scroll bars to adjust contrast
Right now, the maximum value of this image is 3628 and the minimum value of this image is 141.
For example, after changing Max and Min, the maximum value becomes 1329 and the minimum value of this image is 654 When you are moving your mouse around on the image, the data of each pixel will show up above the image. (orange box in the figure above)