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Training for the bioconductor-scp tool #5709

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b3226c1
bioconductor-scp tutorial
KristinaGomoryova Jan 21, 2025
98c25e9
contributors updated
KristinaGomoryova Jan 21, 2025
67f5718
bioconductor-scp link on toolshed
KristinaGomoryova Jan 22, 2025
a8c7984
`Group` tool ID updated
KristinaGomoryova Jan 24, 2025
388a1c4
Merge branch 'main' into scp_training
hechth Jan 28, 2025
486d6c2
typos corrected
KristinaGomoryova Jan 30, 2025
a2bca76
combat citation added
KristinaGomoryova Jan 30, 2025
7735636
[here] link replaced by [on Zenodo]
KristinaGomoryova Jan 30, 2025
9aeed93
Merge branch 'main' into scp_training
hechth Feb 3, 2025
ab13219
SCoPE2 typo corrected
KristinaGomoryova Feb 3, 2025
3928dca
columns specified; comBat highlighted
KristinaGomoryova Feb 3, 2025
6a11546
aggregation description adjusted
KristinaGomoryova Feb 3, 2025
ff290d8
SCOPE2 scheme added
KristinaGomoryova Feb 4, 2025
6940f24
sampleAnnotation columns reordered
KristinaGomoryova Feb 4, 2025
532475c
columns in evidences reordered
KristinaGomoryova Feb 4, 2025
f1f6ef3
normalization of row/cols switched
KristinaGomoryova Feb 4, 2025
d899614
abbr for PCA and UMAP introduced earlier
KristinaGomoryova Feb 4, 2025
66362df
summary hands-on section added
KristinaGomoryova Feb 4, 2025
88a3735
plots and intermediate results described
KristinaGomoryova Feb 4, 2025
afc1adf
initial draft of the data exploration part
KristinaGomoryova Feb 4, 2025
9cd19f3
warning about running tool added
KristinaGomoryova Feb 5, 2025
1505eab
warning boxes added
KristinaGomoryova Feb 5, 2025
0fb84ea
warning box only after 1st hands on
KristinaGomoryova Feb 5, 2025
3c15c85
fix hands on box
pavanvidem Feb 7, 2025
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8 changes: 8 additions & 0 deletions CONTRIBUTORS.yaml
Original file line number Diff line number Diff line change
Expand Up @@ -1401,6 +1401,14 @@ kostrykin:
- elixir-europe
- deNBI

KristinaGomoryova:
name: Kristina Gomoryova
orcid: 0000-0003-4407-3917
joined: 2025-01
email: [email protected]
elixir_node: cz
twitter: kristina_gomory

kxk302:
name: Kaivan Kamali
email: [email protected]
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3 changes: 3 additions & 0 deletions topics/proteomics/tutorials/bioconductor-scp/faqs/index.md
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@@ -0,0 +1,3 @@
---
layout: faq-page
---
150 changes: 150 additions & 0 deletions topics/proteomics/tutorials/bioconductor-scp/tutorial.bib
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# This is the bibliography file for your tutorial.
#
# To add bibliography (bibtex) entries here, follow these steps:
# 1) Find the DOI for the article you want to cite
# 2) Go to https://doi2bib.org and fill in the DOI
# 3) Copy the resulting bibtex entry into this file
#
# To cite the example below, in your tutorial.md file
# use {% cite Batut2018 %}
#
# If you want to cite an online resourse (website etc)
# you can use the 'online' format (see below)
#
# You can remove the examples below

@article{Lin2024,
title = {Review and Practical Guide for Getting Started With Single‐Cell Proteomics},
volume = {25},
ISSN = {1615-9861},
url = {http://dx.doi.org/10.1002/pmic.202400021},
DOI = {10.1002/pmic.202400021},
number = {1–2},
journal = {PROTEOMICS},
publisher = {Wiley},
author = {Lin, Hsien‐Jung L. and Webber, Kei G. I. and Nwosu, Andikan J. and Kelly, Ryan T.},
year = {2024},
month = nov
}

@article{Gatto2023,
title = {Initial recommendations for performing, benchmarking and reporting single-cell proteomics experiments},
volume = {20},
ISSN = {1548-7105},
url = {http://dx.doi.org/10.1038/s41592-023-01785-3},
DOI = {10.1038/s41592-023-01785-3},
number = {3},
journal = {Nature Methods},
publisher = {Springer Science and Business Media LLC},
author = {Gatto, Laurent and Aebersold, Ruedi and Cox, Juergen and Demichev, Vadim and Derks, Jason and Emmott, Edward and Franks, Alexander M. and Ivanov, Alexander R. and Kelly, Ryan T. and Khoury, Luke and Leduc, Andrew and MacCoss, Michael J. and Nemes, Peter and Perlman, David H. and Petelski, Aleksandra A. and Rose, Christopher M. and Schoof, Erwin M. and Van Eyk, Jennifer and Vanderaa, Christophe and Yates, John R. and Slavov, Nikolai},
year = {2023},
month = mar,
pages = {375–386}
}

@article{Ahmad2023,
title = {A review of the current state of single-cell proteomics and future perspective},
volume = {415},
ISSN = {1618-2650},
url = {http://dx.doi.org/10.1007/s00216-023-04759-8},
DOI = {10.1007/s00216-023-04759-8},
number = {28},
journal = {Analytical and Bioanalytical Chemistry},
publisher = {Springer Science and Business Media LLC},
author = {Ahmad, Rushdy and Budnik, Bogdan},
year = {2023},
month = jun,
pages = {6889–6899}
}

@article{Specht2021,
title = {Single-cell proteomic and transcriptomic analysis of macrophage heterogeneity using SCoPE2},
volume = {22},
ISSN = {1474-760X},
url = {http://dx.doi.org/10.1186/s13059-021-02267-5},
DOI = {10.1186/s13059-021-02267-5},
number = {1},
journal = {Genome Biology},
publisher = {Springer Science and Business Media LLC},
author = {Specht, Harrison and Emmott, Edward and Petelski, Aleksandra A. and Huffman, R. Gray and Perlman, David H. and Serra, Marco and Kharchenko, Peter and Koller, Antonius and Slavov, Nikolai},
year = {2021},
month = jan
}

@online{creative-proteomics,
author = {Creative Proteomics},
title = {Principal Component Analysis},
url = {https://www.creative-proteomics.com/services/pca-service.htm},
urldate = {2025-01-21}
}

@article{Phua2022,
title = {Perspectives for better batch effect correction in mass-spectrometry-based proteomics},
volume = {20},
ISSN = {2001-0370},
url = {http://dx.doi.org/10.1016/j.csbj.2022.08.022},
DOI = {10.1016/j.csbj.2022.08.022},
journal = {Computational and Structural Biotechnology Journal},
publisher = {Elsevier BV},
author = {Phua, Ser-Xian and Lim, Kai-Peng and Goh, Wilson Wen-Bin},
year = {2022},
pages = {4369–4375}
}

@article{Zhang2018,
title = {Alternative empirical Bayes models for adjusting for batch effects in genomic studies},
volume = {19},
ISSN = {1471-2105},
url = {http://dx.doi.org/10.1186/s12859-018-2263-6},
DOI = {10.1186/s12859-018-2263-6},
number = {1},
journal = {BMC Bioinformatics},
publisher = {Springer Science and Business Media LLC},
author = {Zhang, Yuqing and Jenkins, David F. and Manimaran, Solaiappan and Johnson, W. Evan},
year = {2018},
month = jul
}

@online{distill-tSNE,
author = {Distill},
title = {How to Use t-SNE Effectively},
url = {https://distill.pub/2016/misread-tsne/},
urldate = {2025-01-21}
}

@online{understanding-umap,
author = {Coenen, Pearce},
title = {Understanding UMAP},
url = {https://pair-code.github.io/understanding-umap/},
urldate = {2025-01-21}
}

@online{understanding-umap2,
author = {Aastha Varma},
title = {Dimensionality Reduction: PCA, t-SNE, and UMAP},
url = {https://medium.com/@aastha.code/dimensionality-reduction-pca-t-sne-and-umap-41d499da2df2},
urldate = {2025-01-21}
}

@online{number-pca-components,
author = {Kevin O'Brien},
title = {https://rpubs.com/DragonflyStats/pca_components_number},
url = {https://rpubs.com/DragonflyStats/pca_components_number},
urldate = {2025-01-21}
}

@article{Goh2017,
title = {Why Batch Effects Matter in Omics Data, and How to Avoid Them},
volume = {35},
ISSN = {0167-7799},
url = {http://dx.doi.org/10.1016/j.tibtech.2017.02.012},
DOI = {10.1016/j.tibtech.2017.02.012},
number = {6},
journal = {Trends in Biotechnology},
publisher = {Elsevier BV},
author = {Goh, Wilson Wen Bin and Wang, Wei and Wong, Limsoon},
year = {2017},
month = jun,
pages = {498–507}
}
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