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| # Cube-based Light-sheet Apparatus for Versatile 3d-imaging Experiments (CLAVE) [](https://github.com/ellerbrock/open-source-badges/) | ||
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| <p align="left"> | ||
| <img src="./images/CLAVE.jpg" width="150"> | ||
| </p> | ||
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| Appropriate technology open source light sheet microscope design, for 3d imaging and high-throughput droplet cytometry. | ||
| This is a modification of the OpenUC2 light sheet design - a project that can be explored by [website](https://openuc2.de) and [GitHub](https://github.com/openUC2/UC2-GIT). This version has been developed as part of a [crowd funded project](https://experiment.com/projects/developing-a-low-cost-high-sensitivity-solution-for-phage-viral-load-detection) on experiment.com by Tobias Wenzel and Benedict Diederich. In the Wenzel Lab, Matías Hurtado has also contributed to the design of several cubes, and the re-design of Inventor designs of [UC2 cubes in OnShape](https://cad.onshape.com/documents/6ad90b1b9211810137d71b1d/w/b1b0cc05a0494bf4bc912f91/e/37e5ce9fc21af2a3e931ec46?configuration=Distances%3D0.03%2Bmeter&renderMode=0&uiState=668f4aab4587f461c0a71ce5). | ||
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| Follow us! [#twitter](https://twitter.com/WenzelLab), [#YouTube](https://www.youtube.com/@librehub), [#LinkedIn](https://www.linkedin.com/company/92802424), [#instagram](https://www.instagram.com/wenzellab/), [#Printables](https://www.printables.com/@WenzelLab), [#LIBREhub website](https://librehub.github.io), [#IIBM website](https://ingenieriabiologicaymedica.uc.cl/en/people/faculty/821-tobias-wenzel) | ||
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| ## Build your own! | ||
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| Please find the detailed [online documentation here](https://wenzel-lab.github.io/light-sheet-microscope/). | ||
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| <p align="left"> | ||
| <img src="./images/light-sheet.png" width="500"> | ||
| </p> | ||
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| <p align="left"> | ||
| <img src="./images/cube-arrangement.png" width="300"> | ||
| </p> | ||
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| Illumination arm: | ||
| 1) Fiber coupler, colimator, 5mm mask, all mounted on one threaded UC2 insert. Fiber coupler and colimator: Thorlabs AD11F with F280FC-A. The 5mm mask was 3d printed to cut the beam to the optimal size for the Powell lense. | ||
| 3) Powell-lense and optional slit | ||
| <p align="left"> | ||
| <img src="./images/Powell_lense_aligner.jpg" height="300"> <img src="./images/adjustable_slit.gif" height="300" width="300"> | ||
| </p> | ||
| 4) cylindrical lense f=150mm (or 100mm in cube 5, or 50mm in cube 6) | ||
| <p align="left"> | ||
| <img src="./images/cylindrical_lense1.jpg" height="300"> | ||
| <img src="./images/cylindrical_lense2.jpg.jpg" height="300"> | ||
| </p> | ||
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| 5) kinematic mirror (optional if using a straight illumination arm or a shorter focal length cylindrical lense) | ||
| 6) empty | ||
| 7) sample holder | ||
| 8) XYZ stage for sample | ||
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| Detection arm: | ||
| 10) 1- 5x Objective | ||
| 2- Focus motor | ||
| 11) 1- Optical filter | ||
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| <p align="left"> | ||
| <img src="./images/filter-holder.jpeg" height="300"> | ||
| </p> | ||
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| 2- Tube-lense and camera | ||
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| <p align="left"> | ||
| <img src="./images/tubelense-double-cube.jpeg" height="300"> | ||
| </p> | ||
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| There is also crontol electronics, a playstation controller, and a computer running Ubuntu | ||
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| ## Contribute | ||
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| This is an open project in the Wenzel Lab in Santiago, Chile. If you have any suggestions to improve it or add any additional functions make a pull-request or [open an issue](https://github.com/wenzel-lab/light-sheet-microscope/issues/new). | ||
| For interactions in our team and with the community applies the [GOSH Code of Conduct](https://openhardware.science/gosh-2017/gosh-code-of-conduct/). | ||
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| ## License | ||
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| [CERN OHL 2W](LICENSE). This project is Open Source Hardware - please acknowledge us when using the hardware or sharing modifications. | ||
| # CLAVE Microscope | ||
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| ## **CLAVE:** Cube-based Light-sheet Apparatus for Versatile 3D-imaging Experiments | ||
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| Appropriate technology open source light sheet microscope design, for 3d imaging and high-throughput droplet cytometry. This is a modification of the OpenUC2 light sheet design - a project that can be explored by the [**website** ](https://openuc2.de/)and [**GitHub**](https://github.com/openUC2/UC2-GIT). This version has been developed as part of a [**crowd-funded project**](https://experiment.com/projects/developing-a-low-cost-high-sensitivity-solution-for-phage-viral-load-detection) on experiment.com by [Tobias Wenzel](https://librehub.github.io/people/tobias_wenzel/index.html) and [Benedict Diederich](https://openuc2.com/author/benedi/). In the Wenzel Lab, [Matías Hurtado](https://librehub.github.io/people/matias_hurtado/index.html) has also contributed to the design of several cubes, and the re-design of Inventor designs of [**UC2 cubes in OnShape.**](https://cad.onshape.com/documents/6ad90b1b9211810137d71b1d/w/b1b0cc05a0494bf4bc912f91/e/37e5ce9fc21af2a3e931ec46?configuration=Distances%3D0.03%2Bmeter&renderMode=0&uiState=668f4aab4587f461c0a71ce5) | ||
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| #Build your own: | ||
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|  | ||
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| ##Navigation: | ||
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| * [Parts Gallery](gallery.md) | ||
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| * [Prepare the pieces](Prepare.md) | ||
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| * [Build your UC2](UC2files.md) | ||
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| --- | ||
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| ##Follow us: | ||
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| * [Twitter](https://twitter.com/WenzelLab), | ||
| * [YouTube](https://www.youtube.com/@librehub>), | ||
| * [LinkedIn](https://www.linkedin.com/company/92802424), | ||
| * [Instagram](https://www.instagram.com/wenzellab/), | ||
| * [Printables](https://www.printables.com/@WenzelLab/models) & [Thingiverse](https://www.thingiverse.com/libre-hub/designs) | ||
| * [LIBREhub website](https://librehub.github.io/), | ||
| * [IIBM website](https://ingenieriabiologicaymedica.uc.cl/en/people/faculty/821-tobias-wenzel) | ||
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| ##Contribute: | ||
| This is an open project in the Wenzel Lab in Santiago, Chile. If you have any suggestions to improve it or add additional functions make a pull-request or [open an issue](https://github.com/wenzel-lab/light-sheet-microscope/issues/new). For interactions in our team and with the community applies the [GOSH Code of Conduct](https://openhardware.science/gosh-2017/gosh-code-of-conduct/). | ||
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| ##License: | ||
| [CERN OHL 2W.](https://github.com/wenzel-lab/light-sheet-microscope/blob/main/LICENSE) This project is Open Source Hardware - please acknowledge us when using the hardware or sharing modifications. |