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add TYGS images to whole-genome workshop section
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widdowquinn committed Jan 19, 2024
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28 changes: 28 additions & 0 deletions references.bib
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Expand Up @@ -581,3 +581,31 @@ @ARTICLE{Edgar2018-cm
copyright = "https://academic.oup.com/journals/pages/about\_us/legal/notices",
language = "en"
}

@ARTICLE{Maiden2013-jh,
title = "{MLST} revisited: the gene-by-gene approach to bacterial
genomics",
author = "Maiden, Martin C J and Jansen van Rensburg, Melissa J and Bray,
James E and Earle, Sarah G and Ford, Suzanne A and Jolley, Keith
A and McCarthy, Noel D",
abstract = "Multilocus sequence typing (MLST) was proposed in 1998 as a
portable sequence-based method for identifying clonal
relationships among bacteria. Today, in the whole-genome era of
microbiology, the need for systematic, standardized descriptions
of bacterial genotypic variation remains a priority. Here, to
meet this need, we draw on the successes of MLST and 16S rRNA
gene sequencing to propose a hierarchical gene-by-gene approach
that reflects functional and evolutionary relationships and
catalogues bacteria 'from domain to strain'. Our gene-based
typing approach using online platforms such as the Bacterial
Isolate Genome Sequence Database (BIGSdb) allows the scalable
organization and analysis of whole-genome sequence data.",
journal = "Nat. Rev. Microbiol.",
publisher = "Springer Science and Business Media LLC",
volume = 11,
number = 10,
pages = "728--736",
month = oct,
year = 2013,
language = "en"
}
13 changes: 12 additions & 1 deletion whole_genome.qmd
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Expand Up @@ -9,18 +9,29 @@ Please ensure that you have downloaded the genome file for your isolate (`isolat
## TYGS

- Go to the [TYGS](https://tygs.dsmz.de/) server

![](assets/images/TYGS/01-tygs.png)

- Click on `Submit your query` (top menu), or `Submit your job` (button) to reach the query page

![](assets/images/TYGS/02-tygs.png)

- Click on the `Browse…` button and navigate to your `isolate_genome.fasta` file to select it for classification.
- Enter your email address in the `Provide contact details` field

![](assets/images/TYGS/03-tygs.png)

- Click on `Submit query` and wait for the results

![](assets/images/TYGS/04-tygs.png)

::: { .callout-warning }
This may take some time (maybe over an hour, depending on server load!), and may not be complete before the end of the workshop.

**Please move on to the next section while you are waiting.**
:::

- When you get the result confirmation email, check the PDF
- **When you get the result confirmation email, inspect the PDF**

::: { .callout-important title="Questions"}
1. What is the predicted taxonomic classification of your isolate's genome?
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