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Ampliseq Version 2.3.2
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@d4straub d4straub released this 27 May 11:14
· 1285 commits to master since this release
2.3.2
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nf-core/ampliseq version 2.3.2 - 2022-05-27

Summary of changes

This release fixes bugs and adds more options to manipulate cutadapt parameters.

Detailed changes

Added

  • #429 - --cutadapt_min_overlap sets cutadapt's global minimum overlap (-O) and --cutadapt_max_error_rate sets cutadapt's global maximum error rate (-e) for trimming primer sequences.
  • #431 - --skip_dada_quality allows to skip quality check with DADA2. This is only allowed when --trunclenf and --trunclenr are set.

Changed

  • #432 - The number of records to sample from a fastq file was decreased from 5e+06 to 5e+04 for plotQualityProfile (DADA2_QUALITY), therefore a smaller subset of reads is sampled for determining --trunlenf and --trunclenr. This should make the process more robust also from larger data sets.

Fixed

  • #428 - Fixed samplesheet sampleID entries, now allows dashes.
  • #433 - Fixed typos and improved documentation layout.
  • #437 - Template update for nf-core/tools version 2.4
  • #439 - Fixed a bug in DADA2_QUALITY process with large number of nucleotides.
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