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7 changes: 6 additions & 1 deletion _pages/plugins/snt/analysis.md
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Expand Up @@ -242,7 +242,7 @@ Delineations allow measuring proportions of reconstructions within other structu
Delineations are described in [Walkthroughs › Delineation Analysis](/plugins/snt/walkthroughs#delineation-analysis).

# Root Angle Analysis
Root angle analysis measures the angular distribution of how far neurites deviate from a direct path to the soma (or root of the neuronal arbor), a functional property that is captured by [Sholl profiles](#sholl-analysis). It quantifies properties such as [balancing factor](./metrics#root-angles-balancing-factor), [centripetal bias](./metrics#root-angles-centripetal-bias), and [mean direction](./metrics#root-angles-mean-direction). It is described in:
Root angle analysis measures the angular distribution of how far neurites deviate from a direct path to the soma (or root of the neuronal arbor), a functional property that is captured by [Sholl profiles](#sholl-analysis) (see also [angular Sholl](./sholl#angular-sholl)). It quantifies properties such as [balancing factor](./metrics#root-angles-balancing-factor), [centripetal bias](./metrics#root-angles-centripetal-bias), and [mean direction](./metrics#root-angles-mean-direction). It is described in:

{% include citation doi='10.1016/j.celrep.2019.04.097' %}

Expand All @@ -263,6 +263,11 @@ The analysis can be performed from the [Analysis menu](/plugins/snt/manual#root-

Growth Analysis provides detailed time-lapse analysis of neuronal patterns and requires traced paths to be matches across time frames, as detailed in. The Analysis is accessed through the [Path Manager](./manual#path-manager)'s [Time-lapse Utilities](./manual#time-lapse-utilities-) menu.

{% capture timelapse-demo%}
For parameter validation and configuration comparisons, load the _Hippocampal neuron (DIC timelapse)_ demo dataset ({% include bc path='File|Load Demo Dataset...' %})
{% endcapture %}
{% include notice icon="info" content=timelapse-demo %}

## Prerequisites

- **Time-matched paths**: Paths must be tagged using the {% include bc path='Match Paths Across Time...'%} command first, so that all paths in the timelapse sequence associated with the same neurite are tagged with a common neurite label, e.g., "{neurite #1}", "{neurite #2}", etc, as described in the [Time-lapse analysis](./walkthroughs#time-lapse-analysis) walkthrough.
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25 changes: 25 additions & 0 deletions _pages/plugins/snt/big-data.md
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@@ -0,0 +1,25 @@
---
title: SNT › Big Data
nav-links: true
nav-title: Big Data
artifact: org.morphonets:SNT
icon: /media/icons/snt.png
forum-tag: snt
update-site: Neuroanatomy
tags: snt,tracing,segmentation,neuroanatomy,big-data
---

{% capture version%}
**This page was last revised for [version 5.0.0](https://github.com/morphonets/SNT/releases)**.
{% endcapture %}
{% include notice content=version %}

## Big Data support

SNTv5 implemented _preliminary_ support for big data. The support remains basic but currently supports the following operations:

| Operation | Status | Details |
|--------------------|-----------------------|---------|
| Tracing operations | Headless support only | A* tracing performed using pre-existing coordinates is fully supported via scripting in headless operations. [Example implementation](https://github.com/AllenNeuralDynamics/neuron-tracing-utils). For interactive tracing, please use [HortaCloud](https://hortacloud.org/): This is SNT's development team recommended tool for tracing Terabyte-size datasets |
| Path optimization | Headless support only | [Optimization of curvatures](./manual#refinefit-), including extraction of radii |
| 3D Visualization | Supported via [sciview](./manual#sciview) and [BigVolumeViewer](./manual#bigvolumeviewer) | Visualization of 3D reconstructions, including [color mappings](./manual#color-mapping-), etc. |
5 changes: 3 additions & 2 deletions _pages/plugins/snt/comp-tools.md
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Expand Up @@ -13,15 +13,16 @@ update-site: Neuroanatomy
| Tool | Description | Comments |
|------|-------------|----------|
| [Spot Spine](/plugins/spot-spine) | Detection and count dendritic spines, segmentation of spine heads, and quantitative morphological measurements of spines | Under active development |
| [BigTrace](/plugins/bigtrace) | Semi-automated tracing in 3D using BigVolumeViewer | Under active development |
| [BigTrace](/plugins/bigtrace) | Semi-automated/automated tracing of unbranched structures using BigVolumeViewer | Under active development |
| [NeuronJ](/plugins/neuronj) | Semi-automated tracing in 2D only | No longer maintained. SNT reads NDF files |


### Other

| Tool | Description |
|------|-------------|
| [HortaCloud](https://hortacloud.org/) | Cloud-based, open-source platform for collaborative reconstruction of long-range projection neurons from whole-brain light microscopy data |
| [HortaCloud](https://hortacloud.org/) | Cloud-based, open-source platform for collaborative reconstruction of long-range projection neurons from whole-brain light microscopy data. This is SNT's development team recommended tool for tracing [Terabyte-size datasets](./big-data) |
| | |
| [BICCN Tools](https://biccn.org/tools) | List of tools supporting NIH's Brain Research through Advancing Innovative Neurotechnologies (BRAIN) Initiative - Cell Census Network (BICCN) |
| [Blue Brain's Morphology Suite](https://github.com/BlueBrain/morphology-suite) | Blue brain's collection of tools (mostly Python) to build, analyze and visualize morphologies. Currently supported by the [Open Brain Institute](https://www.openbraininstitute.org/) |
| [Cajal](https://cajal.readthedocs.io/en/latest/index.html#) | A Python package for the analysis of single-cell morphological data using Gromov-Wasserstein (GW) distances |
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7 changes: 4 additions & 3 deletions _pages/plugins/snt/index.md
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Expand Up @@ -40,11 +40,12 @@ The [source repository](https://github.com/morphonets/SNT) contains more details
/media/plugins/snt/snt-montage-light.png | Quantitative and publication quality visualizations
/media/plugins/snt/snt-montage-dark.png | Data-rich 3D visualizations
/media/plugins/snt/snt-rec-viewer-demo2-dark.gif | [Interactive 3D scenes](/plugins/snt/reconstruction-viewer)
/media/plugins/snt/sholl-analysis-outputs.png | [Sholl](/plugins/snt/analysis#sholl-analysis) and [Strahler](/plugins/snt/analysis#strahler-analysis) analysis
/media/plugins/snt/snt-delineation-analysis2.png | [Delineation analyses](/plugins/snt/walkthroughs#delineation-analysis)
/media/plugins/snt/sholl-analysis-outputs.png | [Sholl](/plugins/snt/#sholl) and [Strahler](/plugins/snt/analysis#strahler) directly from images (bypassing tracing)
/media/plugins/snt/snt-angular-sholl.png | Advanced [Sholl-based quantifications](/plugins/snt/sholl#angular-sholl)
/media/plugins/snt/snt-root-angle-analysis.png | [Root Angle Analysis](/plugins/snt/analysis#root-angle-analysis)
/media/plugins/snt/snt-local-angle-surface-analysis.png | Analysis of [surface angles/arbor orientation](/plugins/snt/metrics#extension-angle)
/media/plugins/snt/snt-delineation-analysis2.png | [Delineation analyses](/plugins/snt/walkthroughs#delineation-analysis)
/media/plugins/snt/snt-growth-analysis.png | [Growth Analysis](/plugins/snt/analysis#growth-analysis)
/media/plugins/snt/snt-root-angle-analysis.png | [Root Angle Analysis](/plugins/snt/analysis#root-angle-analysis)
/media/plugins/snt/snt-ferris-wheel.png | Routines to summarize [innervation patterns](/plugins/snt/analysis#graph-based-analysis)
/media/plugins/snt/graph-viewer-ferris-wheel.png | Routines to summarize data from [projectomes and connectomics](/plugins/snt/analysis#graph-based-analysis)
/media/plugins/snt/snt-2d-histogram.png | Specialized [statistics](/plugins/snt/analysis#statistics)
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1 change: 1 addition & 0 deletions _pages/plugins/snt/key-shortcuts.md
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Expand Up @@ -51,6 +51,7 @@ The [Command Palette](manual#command-palette) ({% include key keys='ctlcmd|Shift
| {% include key key='2' %} | Toggles the second visibility filter: Whether all nodes should be displayed across the Z-stack or just those in nearby Z-slices |
| {% include key key='3' %} | Toggles the third visibility filter: Whether paths from all channels/frames should be displayed or just those in the active channel/frame |
| {% include key key='G' %} | Selects the nearest path to the mouse cursor. Holding {% include key keys='Shift|G' %} adds the path nearest to the mouse cursor to the current list of selected paths. *Mnemonic: <u>G</u>roup paths around cursor.* Note that Paths can only be edited one at a time, and thus {% include key keys='Shift|G' %} is disabled in *Edit Mode* |
| {% include key key='H' %} | Temporarily <u>H</u>ides alls paths/annotations while being pressed |


### Tracing
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3 changes: 2 additions & 1 deletion _pages/plugins/snt/manual.md
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Expand Up @@ -477,7 +477,8 @@ This option assumes [sciview](/plugins/sciview) to be successfully installed. sc
### Big Volume Viewer

Big Volume Viewer (BVV) is the 3D counterpart of [BigDataViewer](https://imagej.net/plugins/bdv) capable of GPU volume rendering of images too large to fit into memory. Currently, support for Big Volume Viewer remains highly experimental.

{% include img align="center" name="BVV integration" src="/media/plugins/snt/snt-bvv.png" caption="BVV integration" %}

### Legacy 3D Viewer

The Legacy 3D Viewer is a functional tracing canvas and allows images to be traced interactively in 3D. However, it relies on libraries that are not actively maintained and _may_ not function reliably during complex tasks. That being said, SNTv5 has implemented several improvements that have restored/improved Legacy 3D Viewer functionality relatively to earlier versions. For usage instructions, see [Tracing using the Legacy 3D Viewer](/plugins/snt/walkthroughs#tracing-in-the-legacy-3d-viewer).
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