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## 📜 About

This repo hosts training materials for the University of Cambridge [Part III Systems Biology course](https://www.sysbiol.cam.ac.uk/Part%20III). More specifically, these are practical exercises for the computational session on flux balance analysis (FBA) using genome-scale metabolic models (GEMs). Most of the exercises are based on the [cobrapy documentation](https://cobrapy.readthedocs.io/en/latest/).
This repo hosts training materials for the University of Cambridge [Part III Systems Biology course](https://www.sysbiol.cam.ac.uk/Part%20III). More specifically, these are practical exercises for the computational session on flux balance analysis (FBA) using genome-scale metabolic models (GEMs). Exercise 1 is based on selected portions of the [cobrapy documentation](https://cobrapy.readthedocs.io/en/latest/).

### Learning outcomes

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- **2.2**: Perform gene essentiality analysis under different conditions
- **2.3**: Case study, simulate the Crabtree effect in yeast

## 🚚 Software requirements

The following packages will already be installed on the provided machines:

* [cobrapy](https://opencobra.github.io/cobrapy/)
* [matplotlib](https://matplotlib.org/stable/)
* [numpy](https://numpy.org/install/)
* [notebook](https://jupyter.org/install#jupyter-notebook)

To install on your own computers use the [pip package manager](https://pip.pypa.io/en/stable/getting-started/).

Using the `requirements.txt` files:

```
$ pip install -r requirements.txt
```

Or from scratch:

```
$ pip install cobra matplotlib numpy notebook
```

## 🛠️ Usage

You will find everything you need for these practical exercises under the `/notebooks` folder. Each tutorial is uploaded as `.html`, `.md`, `.tex`, and `.ipynb` files. As you carry out the exercises using the the python notebooks, you will find the original text and results in the html, markdown, and latex files.
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## 👷 Contributors

* Originally developed by Arianna Basile and Kiran Patil in 2022
* Originally developed by Arianna Basile and Kiran Patil in 2023
* Updated by Francisco Zorrilla and Arianna Basile in 2024


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