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A meta-listing of software, training resources, and more from the Cimini Lab at the Broad Institute Imaging Platform

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Cimini Lab resources

Below are ways to collaborate our lab, pointers to our software and its documentation, and the educational resources and papers we have been a part of. We hope these resources help you out!

This page last updated 2025-Feb-06

Jump to:

Work with us | Software | Educational materials | Papers

Work with us {#collaborate}

Lab website

Office hours link

CellProfiler annual support plan

COBA signup form

Beth's Broad bio page with contact email

Google Summer of Code 2025 Project ideas

Software we make {#software}

Jump to:

CellProfiler | CellProfiler Analyst | Piximi | Distributed Something | Bilayers

CellProfiler {#cellprofiler}

downloads

manual

plugins documentation

tutorials

GitHub

help forum

pipeline suggester

papers - CellProfiler-plugins [2023] / CellProfiler 4 [2021] / CellProfiler 3 [2018]

CellProfiler Analyst {#cpa}

downloads

manual

GitHub

help forum

papers - CellProfiler Analyst 3 [2021]

Piximi {#piximi}

Piximi.app

manual

GitHub

help forum

preprint

Distributed-Something {#ds}

GitHub

paper

Distributed tools - Distributed-CellProfiler / Distributed-Collate / Distributed-DeepProfiler / Distributed-Fiji / Distributed-HelloWorld / Distributed-OMEZarrCreator

Bilayers {#bilayers}

demo app

documentation

GitHub

Dockerhub

Educational materials {#educational}

Jump to:

Educational papers | Things we lead | Major contributors | Minor contributors

Educational papers we wrote {#ed-papers}

The Twenty Questions of Bioimage Object Analysis[2023] / Tool implementation

A biologist’s guide to planning and performing quantitative bioimaging experiments[2023]

Creating and troubleshooting microscopy analysis workflows: Common challenges and common solutions[2024]

A postdoctoral training program in bioimage analysis[2024]

Things we lead {#ed-lead}

Lab website

Lab paper page

Current lab preprints (from Beth's CV)

Imaging Platform Blog

YouTube tutorials

Office hours link

The Scientific Community Image Forum - Forum.image.sc / Paper - Scientific Community Image Forum: A discussion forum for scientific image software[2019]

COBA website

Bioimaging Guide - English / Spanish / Portuguese / Czech

The Image Based Profiling Handbook

The Cell Painting Gallery (image repository) - AWS RODA Website / Explore on Quilt / Paper - Cell Painting Gallery: an open resource for image-based profiling[2024]

Things we are major (but far from sole) contributors to {#ed-major}

Global Bioimage Analysts' Society (GloBIAS) Global Registries - For bioimage analysts / For bioimage analysis trainers

Virtual I2K Meeting - 2023 edition / 2024 edition

Things we contributed to (but others led) {#ed-minor}

Online guide for checklists for imaging and image analysis / Paper - Community-developed checklists for publishing images and image analysis [2023]

Metrics Reloaded online metric chooser / Paper 1 - Metrics reloaded: recommendations for image analysis validation[2024] / Paper 2 - Understanding metric-related pitfalls in image analysis validation[2024]

Microtutor imaging education platform

Papers you might find useful {#papers}

Jump to:

Protocols | Reviews | Community Surveys | Opinions/Perspectives

Protocols {#protocols}

ImageJ and CellProfiler: Complements in Open-Source Bioimage Analysis[2020]

Interpreting image-based profiles using Morpheus and single-cell visualization[2023]

Optimizing the Cell Painting assay for image-based profiling[2023]

Reviews {#reviews}

Open-source deep-learning software for bioimage segmentation[2021]

Defining the boundaries: challenges and advances in identifying cells in microscopy images[2024]

Community Surveys {#surveys}

2020 BioImage Analysis Survey: Community experiences and needs for the future[2021]

Bridging Imaging Users to Imaging Analysis - A community survey[2023]

Opinions/Perspectives {#opinions}

Moving beyond the desktop: Prospects for Practical Bioimage Analysis via the Web[2023]

Building momentum through networks: Bioimaging across the Americas[2024]

The Crucial Role of BioImage Analysts in Scientific Research and Publication[2024]

A Call for FAIR and Open-Access Training Materials to advance Bioimage Analysis (preprint)[2024]

Harmonizing the Generation and Pre-publication Stewardship of FAIR Image Data (preprint)[2024]

Enabling Global Image Data Sharing in the Life Sciences (preprint)[2024]

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