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# Multi-intron splicing order

This repository contains the scripts necessary to compute multi-intron splicing order from nanopore sequencing data, as performed in the manuscript "Pre-mRNA splicing order is predetermined and maintains splicing fidelity across multi-intronic transcripts"
This repository contains the scripts necessary to compute multi-intron splicing order from nanopore sequencing data, as performed in the manuscripts "Pre-mRNA splicing order is predetermined and maintains splicing fidelity across multi-intronic transcripts" (Choquet et al. Nat Struct Mol Biol 2023) and "Genetic regulation of nascent RNA maturation revealed by direct RNA nanopore sequencing" (Choquet et al. bioRxiv 2024).

![image](https://user-images.githubusercontent.com/15146555/213518357-7d7b46ed-4ce7-45de-b163-e826ccd44606.png)