Rapidly generate maximum intensity projections (MIPs) from an image directory of multi-colour .tif images
How to use:
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Download ImgMax Here. Click the "download raw" button. Just unzip and run. Keep the .exe in the folder.
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Start ImgMax and browse to your directory of images
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Enter the regular expression (RegEx) required to extract the metadata from your image file names. Hint: Use https://regex101.com/ to help you create this. Example Below:
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Click "Check" and make sure that each column is correctly extracting the appropriate file name components. Double check for odd use-cases (e.g. A1 vs A10 may be difficult).
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Select the single element which defines your multi-colour channels used to obtain the images . This is important so you don't merge images with different channels (e.g. Brightfield, DAPI, FITC, Cy3, Cy5) together.
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Select the elements which define unique image groups. In this instance it's 'well' and 'field'. Tip: If you were to only select 'well' you would accidentally merge all images in each well together and ruin the run.
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If done correctly ImgMax will rapidly create a MIP of each image group and channel while saving the new maximum_projection directory next to the original image directory.
