- Log on to cluster and create project repository with the following folders:
- ./fastq_files
- ./outputs
- Submit sbatch job for 'trimgalore.sh'
- Submit sbatch job for each bt2_sam script:
- bt2_sam_09
- bt2_sam_10
- bt2_sam_11
- bt2_sam_12
- bt2_sam_15
- bt2_sam_16
- export files from outputs folder on cluster to local machine
- upload files to IGV and examine each locus (IFNB1, IFIT2, ISG15)
- Run HighThroughputSeq_Group5.R in RStudio (change paths to local directory)