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Update find-required-raw-data-files.md
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kbeutel authored Feb 29, 2024
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9 changes: 8 additions & 1 deletion GetStarted/find-required-raw-data-files.md
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Expand Up @@ -28,10 +28,17 @@ Genvisis requires appropriately formatted raw data files.

The final report files must include several columns: SNP name, Sample ID, GC score, Allele1 - AB, Allele2 - AB, X, and Y. Two optional but helpful columns are B allele frequency and Log R ratio.


Note that, for any of these options, the Illumina manifest file must contain probe sequences for Genvisis to be able to run the **[Run Marker BLAST Annotation](../#/documentation/RunTheGenvisisWorkflow--run-marker-blast-annotation-illumina)** workflow step. Generally it is possible to find a manifest file that contains probe sequences. We recommend contacting Illumina if you need assistance finding a manifest file that contains probe sequences.

### Affymetrix Data (Affy6/Axiom)
For Affymetrix data, Genvisis requires the following:
- **.CEL** files (one per sample)
- Affymetrix Power Tools
- Affymetrix Annotation file (*.annot.csv) aka Manifest (Genvisis comes with **GenomeWideSNP_6.na35.annot.csv**, which works for most Affymetrix projects)

Optionally, you can include an Affymetrix Probe Set file (***.probe_tab**), which can be found inside Affymetrix Power Tools (**/CD\_GenomeWideSNP\_6\_rev3/Full/GenomeWideSNP\_6/LibFiles/GenomeWideSNP\_6.probe\_tab**).
Optionally, you can include an Affymetrix Probe Set file (***.probe_tab**). This file is required for the **[Run Marker BLAST Annotation](../#/documentation/RunTheGenvisisWorkflow--run-marker-blast-annotation-affymetrix)** step of the Genvisis workflow.

The Affy6 probe set file is saved in the [Genvisis resources directory](../#/documentation/GetStarted--resources-directory).

ThermoFisher no longer shares Affymetrix probe sequences, so probe set files for Axiom arrays may be unavailable.

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