This repo contains four scripts, namely script 1-4, which present the workflow for analyzing scRNA-seq data, integrating scRNA-seq and Visium spatial transcriptomics data, making plots shown in the Figure 2 of the related paper, and preparing supplementary figures, respectively. All data are collected from stroke and uninjured mouse cortices and analyzed using Seurat package. Through integrated single-cell and spatial transcriptomics data analysis, the heterogeneity of astrocyte states post-stroke is revealed.
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