Add improved liftover pipeline (using bcftools +liftover) #1165
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Description
In response to ticket #6088.
This PR re-implements the Remapping Nextflow pipeline using
bcftools +liftover(freeseek/score plug-in) instead ofCrossMap.modules/bcftools_liftover.nf— new module that mirrors the oldcrossmap.nf(input:*.vcf, outputs:*.unmap,_report.txt).-
bcftools +liftoverruns inside the docker image fairbrot/bcftools-liftover:latest (bcftools 1.20 + plug-ins).Use case
bcftools +liftoverperforms better on a number of metrics thanCrossMap- see ticket comments for detailed benchmarking info summarised from the manuscript.Testing
I benchmarked the older
Remappingpipeline (usingCrossMap) against this one.Report coming out of this pipeline (
liftover):Report coming out of
RemappingpipelineAs you can see,
Remappingfailed to lift over 35,905 variants, whereasliftoveronly failed to lift over 2,099 variants.