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@@ -14,7 +14,7 @@ contact [John Vivian]([email protected]). | |
### Appendix | ||
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- [Dependencies and Installation](#dependencies-and-installation) | ||
- [Quickstart](#general-usage) | ||
- [Quickstart](#quickstart) | ||
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For detailed information and troubleshooting, see the [Wiki](https://github.com/BD2KGenomics/toil-rnaseq/wiki) | ||
- [Workflow Inputs](https://github.com/BD2KGenomics/toil-rnaseq/wiki/Workflow-Inputs) | ||
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@@ -37,11 +37,6 @@ This workflow takes RNA sequencing reads (fastq / BAM) as input and outputs the | |
│ ├── fusion.txt | ||
│ └── run_info.json | ||
├── QC | ||
│ ├── bamQC | ||
│ │ ├── readDist.txt | ||
│ │ ├── readDist.txt_PASS_qc.txt | ||
│ │ ├── rnaAligned.out.md.sorted.geneBodyCoverage.curves.pdf | ||
│ │ └── rnaAligned.out.md.sorted.geneBodyCoverage.txt | ||
│ ├── fastQC | ||
│ │ ├── R1_fastqc.html | ||
│ │ ├── R1_fastqc.zip | ||
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@@ -62,9 +57,9 @@ This workflow takes RNA sequencing reads (fastq / BAM) as input and outputs the | |
├── rsem_genes.results | ||
└── rsem_isoforms.results | ||
``` | ||
If the user selects options such as `save-star-bam`, or `wiggle`, additional files will appear in the output directory: | ||
If the user selects options such as `save-bam`, or `wiggle`, additional files will appear in the output directory: | ||
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- SAMPLE.sorted.bam OR SAMPLE.sortedByCoord.md.bam if `bamQC` step is enabled. | ||
- SAMPLE.sorted.bam | ||
- SAMPLE.wiggle.bg | ||
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The output tarball is prepended with the unique name for the sample (e.g. SAMPLE.tar.gz). | ||
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kwargs = dict( | ||
name='toil-rnaseq', | ||
version=version, | ||
description="UC Santa Cruz Computational Genomics Lab's Toil-based RNA-seq pipeline", | ||
description="UC Santa Cruz Computational Genomics Lab's Toil-based RNA-seq workflow", | ||
author='UCSC Computational Genomics Lab', | ||
author_email='[email protected]', | ||
url="https://github.com/BD2KGenomics/toil-lib", | ||
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package_dir={'': 'src'}, | ||
packages=find_packages('src'), | ||
entry_points={ | ||
'console_scripts': ['toil-rnaseq = toil_rnaseq.rnaseq_cgl_pipeline:main', | ||
'console_scripts': ['toil-rnaseq = toil_rnaseq.toil_rnaseq:main', | ||
'toil-rnaseq-inputs = toil_rnaseq.input_generation:main']}) | ||
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setup(**kwargs) | ||
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print("\n\n" | ||
"Thank you for installing the UC Santa Cruz Computuational Genomics Lab RNA-seq pipeline\n" | ||
"If you want to run this Toil-based pipeline on a cluster in a cloud, please install Toil\n " | ||
"Thank you for installing the UC Santa Cruz Computuational Genomics Lab RNA-seq workflow\n" | ||
"If you want to run this Toil-based workflow on a cluster in a cloud, please install Toil\n " | ||
"with the appropriate extras. For example, To install AWS/EC2 support for example, run" | ||
"\n\n" | ||
"pip install toil[aws,mesos]%s" | ||
"\n\n" | ||
"For more information, please refer to Toil's documentation:\n" | ||
"http://toil.readthedocs.io/en/latest/installation.html") | ||
"https://toil.readthedocs.io") |
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