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Update doc for mesh synch
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AlexWeinreb committed Jun 5, 2023
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Expand Up @@ -62,10 +62,10 @@ <h2>Principle</h2>
<p>This script has a built-in description of worm development, with stage names and durations. It will draw a timeline predicting what stage the worms have at a given time.</p>
<h3>Synchronization method</h3>
<p>Two methods to synchronize worms are available: via an egg-lay or via L1 arrest. For an egg-lay, gravid adults are placed on plates and allowed to lay eggs for a defined period of time (typically 1h), then removed. That way, all the eggs on the plate will have approximately the same age. Another way to synchronize larger populations of worms is to bleach a large population of gravid adults. The bleach kills all larvae and adults, while embryo survive. The eggs are then placed in a liquid medium without food and allowed to hatch. The larval population becomes L1 arrested due to the lack of food, and will resume their development once plated on food. Protocols for egg-lay and bleach/L1 arrest have been described elsewhere (e.g. <a href="https://dx.doi.org/10.3791/4019">Porta-de-la-Riva <em>et al.</em>, JoVE, 2012</a>).</p>
<p>Three methods to synchronize worms are available: via an egg-lay, L1 arrest, or mesh synchronization. For an egg-lay, gravid adults are placed on plates and allowed to lay eggs for a defined period of time (typically 1h), then removed. That way, all the eggs on the plate will have approximately the same age. Another way to synchronize larger populations of worms is to bleach a large population of gravid adults. The bleach kills all larvae and adults, while embryo survive. The eggs are then placed in a liquid medium without food and allowed to hatch. The larval population becomes L1 arrested due to the lack of food, and will resume their development once plated on food. For mesh synchronization, gravid worms are bleached, the eggs placed on a mesh of appropriate size, and hatching larvae are allowed to crawl through. Protocols for egg-lay and bleach/L1 arrest have been described elsewhere (e.g. <a href="https://dx.doi.org/10.3791/4019">Porta-de-la-Riva <em>et al.</em>, JoVE, 2012</a>).</p>
<h3>Stage durations</h3>
<p>If the checkbox "L1 arrest" is unchecked, the developmental timing is defined based on the description by <a href="https://doi.org/10.1016/0012-1606(76)90119-6">Byerly, Cassada and Russel (Dev. Biol. 1976)</a>. If the checkbox "L1 arrest" is checked, the developmental timing is the one defined in <a href="https://dx.doi.org/10.3791/4019">Porta-de-la-Riva <em>et al.</em>, JoVE, 2012</a>.</p>
<p>In "egg-lay" synchronization, the developmental timing is defined based on the description by <a href="https://doi.org/10.1016/0012-1606(76)90119-6">Byerly, Cassada and Russel (Dev. Biol. 1976)</a>. In "L1 arrest" synchronization, the developmental timing is the one defined in <a href="https://dx.doi.org/10.3791/4019">Porta-de-la-Riva <em>et al.</em>, JoVE, 2012</a>. In "mesh" synchronization, the developmental timing is that described by <a href="https://doi.org/10.1016/0012-1606(76)90119-6">Byerly, Cassada and Russel (Dev. Biol. 1976)</a>, but with L1 stage starting immediately.</p>
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