Questions related to Relax analysis

[T-164321]

Dear Author,
First of all, thank you for providing us with such an excellent software tool. During its use, I have encountered several questions and would appreciate your guidance:

1. Are there any specific requirements for the tree file when performing Relax analysis? I currently have three types of trees: one with divergence times, one with branch lengths (phylogenetic tree), and one with only topological structure. (This is because I obtained different results using the tree with divergence times and the tree with branch lengths.)
2. I have selected 14 species as test branches, but I am not sure if the {FG} display is correct. (The tree file provided includes annotations.)
3. The link contains the code I used, and I am not certain whether it is correct.
   I have stored all relevant information in the link provided. Could you please take a look and help me with these questions? I look forward to your reply.

tissue.txt