Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Tacox plugin: Sequence mismatches on multiple scaffold on random sequence #123

Open
elija-feigl opened this issue Jul 18, 2024 · 0 comments

Comments

@elija-feigl
Copy link
Contributor

Affects:
tacox plugin:
Cadnano design files with multiple scaffold strands, double stranded sections that use the scaffold

Problem:

  • It is only possible to select a single scaffold sequence.
  • either if a single sequence is selected or the random sequence is used
    the sequences of the other scaffold bases will be completely random, not matching the staple strands (see picture: first scaffold with correct pairs (green), second scaffold with mismatches (red)
Screenshot 2024-07-18 at 14 51 17

Expected behaviour:
the tacox plugin should not silently create mismatching duplexes.

  • either assign matching random sequences for other then the first scaffold
  • or disallowing designs in the multiple scaffolds altogether
Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

1 participant