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Copy file name to clipboardExpand all lines: Alignment_of_laser.md
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This will need to be done twice:
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- A rough alignment before the refractive optics are installed &
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- A painstakingly detailed alignment after the refractive optics are
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installed
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- A painstakingly detailed alignment after the refractive optics are installed
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##### Objects needed
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@@ -16,35 +15,24 @@ This will need to be done twice:
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##### Tools needed
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- 1x 1.5 mm hex key/driver
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- Nx Just a little [patience](http://youtu.be/SYzraMwM8n0?t=10s)
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- Nx Just a little [patience](https://youtu.be/SYzraMwM8n0?t=10s)
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##### How to do a laser alignment of the OpenSPIM's illumination axis
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<b>`Take off all jewelry (wedding bands, watches and anything shiny)!`</b>
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<b>Take off all jewelry (wedding bands, watches and anything shiny!</b>
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**Before** the refractive optics are installed:
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Power on laser. Roughly rotate, and adjust the distances to, all of the
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installed mirror mounts so that the laser will reflect off of the middle
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of the mirror surface of each assembly into the back of the illumination
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objective. Then put both laser alignment disk assemblies at each end of
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the 300 mm dovetail rail, finely center the laser through the middle of
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the both assemblies holes in their respective ground glass disks from
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the mirrors on the rail to the mirror on the post. Do the same on the
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150 mm dovetail rail between the Ø1" mirror mount on the post and the
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center of the back of the illumination objective lens.
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Power on laser. Roughly rotate, and adjust the distances to, all of the installed mirror mounts so that the laser will reflect off of the middle of the mirror surface of each assembly into the back of the illumination objective. Then put both laser alignment disk assemblies at each end of the 300 mm dovetail rail, finely center the laser through the middle of the both assemblies holes in their respective ground glass disks from the mirrors on the rail to the mirror on the post. Do the same on the 150 mm dovetail rail between the Ø1" mirror mount on the post and the center of the back of the illumination objective lens.
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**After** the refractive optics are installed:
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Make sure that the laser is going through the middle of all of the
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installed refractive optic lenses. Use an alignment disk at the end of
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the dovetail rail (in relation to the laser light path) to verify that
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the laser is still in line with the correct axis.
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Make sure that the laser is going through the middle of all of the installed refractive optic lenses. Use an alignment disk at the end of the dovetail rail (in relation to the laser light path) to verify that the laser is still in line with the correct axis.
- 3x ThorLabs part # [POLARIS-K1](https://www.thorlabs.com/thorProduct.cfm?partNumber=POLARIS-K1) Ø1" (Ø25.4 mm) PolarisTM Low Drift Kinematic Mirror Mount
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##### Tools needed
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- 1x 2 mm hex key/driver
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##### How to install the mirrors into their respective mirror mounts
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Take both components out of their respective boxes and put the mirror
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inside the mount with its reflective side facing the front, then tighten
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the setscrew to hold the mirror firmly in place.
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*Note that our OpenSPIM prototype system in Dresden uses only the larger
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mirrors.*
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Take both components out of their respective boxes and put the mirror inside the mount with its reflective side facing the front, then tighten the setscrew to hold the mirror firmly in place.
- 2x ThorLabs part # [DG05-1500-H1-MD](https://www.thorlabs.de/thorProduct.cfm?partNumber=DG05-1500-H1-MD) Ø1/2" (Ø12.7 mm) SM05-Mounted Frosted Glass Alignment Disk with Ø1 mm Hole <b><i>threads into</i></b>
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- 2x ThorLabs part # [LMR05/M](https://www.thorlabs.de/thorProduct.cfm?partNumber=LMR05/M) Ø1/2" (Ø12.7 mm) lens mount
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##### Tools needed
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- none
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##### Directions
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Take both components out of their respective boxes and screw the mounted
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alignment disk's external threading into the lens mount's internal
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threading. Same as last step, watch the video to get a good idea how to
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do it.
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Take both components out of their respective boxes and screw the mounted alignment disk's external threading into the lens mount's internal threading. Same as last step, watch the video to get a good idea how to do it.
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#### Ø1" cylindrical lens into indexed rotating mount
- 1x SM1 Spanner Wrench (ThorLabs part # [SPW602](https://www.thorlabs.de/thorProduct.cfm?partNumber=SPW602) or
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[SPW606](https://www.thorlabs.de/thorProduct.cfm?partNumber=SPW606) will work well)
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- 1x 1.3 mm hex key/driver
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##### How to assemble the cylindrical lens system
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Take both components out of their respective boxes, and after taking the
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Ø1" external threaded ring out, put the lens inside the mount with its
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arrow on the banding pointing in the direction of the light path. Use
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the SM1 Spanner Wrench to put the ring back into the threading and
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tighten until the lens does not move.
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Take both components out of their respective boxes, and after taking the Ø1" external threaded ring out, put the lens inside the mount with its arrow on the banding pointing in the direction of the light path. Use the SM1 Spanner Wrench to put the ring back into the threading and tighten until the lens does not move.
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Align the rotation using a 1.3 mm hex key/driver by loosening the two
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screws on the front face of the rotational mount and rotate the face
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plate (not the mount) until it is in the correct position relative to
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the orientation of the cylindrical lens. Tighten the two front face
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screws down one the desired position is reached.
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Align the rotation using a 1.3 mm hex key/driver by loosening the two screws on the front face of the rotational mount and rotate the face plate (not the mount) until it is in the correct position relative to the orientation of the cylindrical lens. Tighten the two front face screws down one the desired position is reached.
Put both O-rings into their places in the sample chamber where the objectives will go. Attach the acrylic sample chamber to the metallic chamber base using the two large M6 capscrews so that the round wholes in each piece face each other. Next screw the illumination and detection objective into the machined objective holder rings. Now orient the chamber so that the acrylic face of the chamber without the opening is facing you and the openings are to your left and away from you. Push the 10x/0.3 illumination objective though the metal and the O-ring-equipped acrylic holes. It may require considerable force, it is recommended to moisten the O-rings beforehand. Rotate the objective so that the three objective holder base threaded holes are aligned with the holes in the metallic chamber base and the flat part of the objective ring is flush with the top of the chamber base. Use three M3 x 8 mm screws to attach the objective to the chamber base as tightly as possible. Repeat the same procedure for the 20x/0.5 detection objective.
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{% include video id="Vuj_ZnfzmUg" width="400" height="243" caption="Assembly SPIM chamber" %}
##### How to assemble the OpenSPIM 4D positioning system
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Install the ball bearing system into the end of the sample arm where the
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hole is drilled, make the hole just a few 10's of microns smaller than
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the outside diameter of the ball bearing system and that it is flush
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with the bottom face of the sample arm.
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Then attach the arm to the system by aligning the holes on the arm that
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will fit the capscrew heads into them with the M3 threaded holes in the
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plate attached to the Y motor.
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Install the ball bearing system into the end of the sample arm where the hole is drilled, make the hole just a few 10's of microns smaller than the outside diameter of the ball bearing system and that it is flush with the bottom face of the sample arm.
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Attach the motor pulley to the motor by tightening it down with a M2.5
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set screw to the motor shaft, and drop the sample pulley into the 12 mm
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ball bearing hole on the sample arm. Finally use a rubber band or large
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diameter small gauge o-ring to go around both pulleys.
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Then attach the arm to the system by aligning the holes on the arm that will fit the capscrew heads into them with the M3 threaded holes in the plate attached to the Y motor.
Attach the motor pulley to the motor by tightening it down with a M2.5 set screw to the motor shaft, and drop the sample pulley into the 12 mm ball bearing hole on the sample arm. Finally use a rubber band or large diameter small gauge o-ring to go around both pulleys.
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## [ Next steps](Install_assembled_ThorLabs_components_on_rail_carriers"wikilink")
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{% include video id="lAon3pz5_cw" width="400" height="243" caption="Connecting the sample arm" %}
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Go back to [Step by step assembly](Step_by_step_assembly"wikilink")
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## [ Next step](Install_assembled_ThorLabs_components_on_rail_carriers)
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[1](Category:Assembly"wikilink")
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Go back to [Step by step assembly](Step_by_step_assembly)
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