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README.md

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@@ -12,21 +12,7 @@ OpenPipeline QC contains an extensible workflow for the generation of a stand-al
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### 1. QC Metrics Calculation
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The workflow calculates extensive ingestion QC metrics, including:
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**Observation level metrics:**
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- `total_{var_type}_by_{expr_type}` - E.g. "total_genes_by_counts". Number of genes with positive counts in a cell.
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- `total_{expr_type}` - E.g. "total_counts". Total number of counts for a cell.
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- `pct_{expr_type}_in_top_{n}_{var_type}` - E.g. "pct_counts_in_top_50_genes". Cumulative percentage of counts for 50 most expressed genes in a cell.
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- `total_{expr_type}_{qc_var}` - E.g. "total_counts_mito". Total number of counts for variables in qc_vars.
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- `pct_{expr_type}_{qc_var}` - E.g. "pct_counts_mito". Proportion of total counts for a cell which are mitochondrial.
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**Variable level metrics:**
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- `total_{expr_type}` - E.g. "total_counts". Sum of counts for a gene.
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- `n_genes_by_{expr_type}` - E.g. "n_genes_by_counts". The number of genes with at least 1 count in a cell.
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- `mean_{expr_type}` - E.g. "mean_counts". Mean expression over all cells.
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- `n_cells_by_{expr_type}` - E.g. "n_cells_by_counts". Number of cells this expression is measured in.
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- `pct_dropout_by_{expr_type}` - E.g. "pct_dropout_by_counts". Percentage of cells this feature does not appear in.
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The workflow calculates extensive ingestion QC metrics at both the observation and feature levels. **Observation-level metrics** include total counts per cell, gene detection rates, mitochondrial and ribosomal gene percentages, and expression concentration in top genes. **Feature-level metrics** cover gene expression summaries such as total counts per gene, detection rates across cells, mean expression levels, and dropout percentages.
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**Spatial data metrics:**
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For spatial data, the workflow also surfaces segmentation metrics.

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