All notable changes to this project will be documented in this file.
The format is based on Keep a Changelog and this project adheres to Semantic Versioning.
- #671 - New
publishDirMode
param and docs - #673, #675, #676 - Profiles for BinAC and CFC clusters in Tübingen
- #679 - Add container for
CreateIntervalBeds
- #692, #697 - Add AWS iGenomes possibilities (within
conf/igenomes.conf
) - #694 - Add monochrome and grey logos for light or dark background
- #698 - Add btb profile for munin server
- #702 - Add font-ttf-dejavu-sans-mono
2.37
and fontconfig2.12.6
to container
- #678 - Changing VEP to v92 and adjusting CPUs for VEP
- #663 - Update
do_release.sh
script - #671 - publishDir modes are now params
- #677, #698, #703 - Update docs
- #679 - Update old awsbatch configuration
- #682 - Specifications for memory and cpus for awsbatch
- #693 - Qualimap bamQC is now ran after mapping and after recalibration for better QC
- #700 - Update GATK to
4.0.9.0
- #702 - Update FastQC to
0.11.8
- #705 - Change
--TMP_DIR
by--tmp-dir
for GATK4.0.9.0
BaseRecalibrator - #706 - Update TravisCI testing
- #665 - Input bam file now has always the same name (whether it is from a single fastq pair or multiple) in the MarkDuplicates process, so metrics too
- #672 - process
PullSingularityContainers
frombuildContainers.nf
now expect a file with the correct.simg
extension for singularity images, and no longer the.img
one. - #679 - Add publishDirMode for
germlineVC.nf
- #700 - Fix #699 missing DP in the FORMAT column VCFs for MuTect2
- #702 - Fix #701
- #705 - Fix #704
- #613 - Add Issue Templates (bug report and feature request)
- #614 - Add PR Template
- #615 - Add presentation
- #616 - Update documentation
- #620 - Add
tmp/
to.gitignore
- #625 - Add
pathfindr
as a submodule - #639 - Add a complete example analysis to docs
- #635 - To process targeted sequencing with a target BED
- #640, #642 - Add helper script for changing version number
- #608 - Update Nextflow required version
- #616 - Update CHANGELOG
- #615 - Use
splitCsv
instead ofreadlines
- #621, #638 - Improve install script
- #621, #638 - Simplify tests
- #627, #629, #637 - Refactor docs
- #629 - Refactor config
- #632 - Use 2 threads and 2 cpus FastQC processes
- #637 - Update tool version gathering
- #638 - Use correct
.simg
extension for Singularity images - #639 - Smaller refactoring of the docs
- #640 - Update RELEASE_CHECKLIST
- #642 - Update conda channel order priorities
- #642 - MultiQC 1.5 -> 1.6
- #642 - Qualimap 2.2.2a -> 2.2.2b
- #642 - VCFanno 0.2.8 -> 0.3.0
- #642 - VCFtools 0.1.15 -> 0.1.16
- #555 -
snpEff
output intoVEP
- #556 -
Strelka
Best Practices - #563 - Use
SnpEFF
reports inMultiQC
- #568 -
VCFTools
processRunVcftools
for QC - #574, #580 - Abstracts for NPMI, JOBIM and EACR25
- #577 - New repository for testing: Sarek-data
- #595 - New library
QC
for functionsbamQC
,bcftools
,samtoolsStats
,vcftools
,getVersionBCFtools
,getVersionGATK
,getVersionManta
,getVersionSnpEFF
,getVersionStrelka
,getVersionVCFtools
,getVersionVEP
- #595 - New Processes
GetVersionBCFtools
,GetVersionGATK
,GetVersionManta
,GetVersionSnpEFF
,GetVersionStrelka
,GetVersionVCFtools
,GetVersionVEP
- #595 - new Python script
bin/scrape_tool_versions.py
inspired by @ewels and @apeltzer - #595 - New QC Process
RunVcftools
- #596 - New profile for BinAC cluster
- #597 - New function
sarek_ascii()
inSarekUtils
- #599, #602 - New Process
CompressVCF
- #601, #603 - Container for GATK4
- #606 - Add test data as a submodule from
Sarek-data
- #608 - Add documentation on how to install Nextflow on
bianca
- #557, #583, #585, #588 - Update help
- #560 - GitHub langage for the repository is now
Nextflow
- #561 -
do_all.sh
build only containers for one genome reference (defaultGRCh38
) only - #571 - Only one container for all QC tools
- #582, #587 - Update figures
- #595 - Function
defineDirectoryMap()
is now part ofSarekUtils
- #595 - Process
GenerateMultiQCconfig
replace by functioncreateMultiQCconfig()
- #597 - Move
checkFileExtension()
,checkParameterExistence()
,checkParameterList()
,checkReferenceMap()
,checkRefExistence()
,extractBams()
,extractGenders()
,returnFile()
,returnStatus()
andreturnTSV()
functions toSarekUtils
- #597 -
extractBams()
now takes an extra parameter. - #597 - Replace depreciated operator
phase
byjoin
. - #597 - Reduce data footprint for Process
CreateRecalibrationTable
- #599 - Merge is tested with
ANNOTATEALL
- #604 - Synching
GRCh38
wgs_calling_regions
bedfiles - #607 - Update to GATK4
- #607 - One container approach
- #608 - Update Nextflow required version
- #616 - Update CHANGELOG
- #617 - Replace depreciated $name syntax with withName
- #560 - Display message for
repository
andcontainerPath
- #566 -
slurmDownload
profile - #579, #584 -
Manta
output reorganized after modification forStrelka Best Practices
process - #585 - Trace file is plain txt
- #590, #593 - Fix Singularity installation in Travis CI testing
- #598, #601 - Fixes for Python script
selectROI.py
to work with CLC viewer
- #607 - Remove Mutect1
- basic wrapper script
- Abstract, posters and figures
- ROI selector and FreeBayes sanitizer scripts
- New logo and icon for the project
- check for existing tumor/normal channel
SarekUtils
withcheckParams()
,checkParameterList()
,checkParameterExistence()
andisAllowedParams()
functions- some
runOptions
fordocker
(prevent some user right problem) - This
CHANGELOG
CAW
is nowSarek
- Dissect Workflow in 5 new scripts:
annotate.nf
,main.nf
,germlineVC.nf
,runMultiQC.nf
andsomaticVC.nf
report.html
,timeline.html
andtrace.html
are generated inReports/
--version
is now used to define the workflow version- most params are now defined in the base.config file instead of in the scripts
- update RELEASE_CHECKLIST.md
checkParams()
,checkParameterList()
,checkParameterExistence()
andisAllowedParams()
in script functions are now called withinSarekUtils
nf_required_version
is nowparams.nfRequiredVersion
- in
buildReferences.nf
script, channels now begin bych_
, and files byf_
- use
PublishDir mode: 'link'
instead ofcopy
directoryMap
now containsparams.outDir
- #539 - use Nextflow support of scratch
- reordered Travis CI tests
- update documentation
MultiQC
version in container from v1.4
to v1.5
vepgrch37
container base image fromrelease_90.6
torelease_92
vepgrch38
container base image fromrelease_90.6
torelease_92
VEP
version in containers from v90
to v91
nucleotidesPerSecond
is nowparams.nucleotidesPerSecond
- default
params.tag
is nowlatest
instead of current version, so --tag needs to be specified with the right version to be sure of using thecontainers
corresponding
standard
profileuppmax-localhost.config
file
scripts/skeleton_batch.sh
- old data and tsv files
- UPPMAX directories from containers
--step
inannotate.nf
,germlineVC.nf
andsomatic.nf
- some
runOptions
for Singularity (binding not needed anymore on UPPMAX) download
profile
- Zenodo for DOI
- Delivery README
- Document use of the
--sampleDir
option - Contributing Guidelines
- Issue Templates
- Release Checklist
--outDir
awsbatch
profileaws-batch.config
config file--noBAMQC
params (failing sometimes on Bianca)
- Update
Nextflow
to0.26.0
(new fancy report + AWS Batch) - Extra time on Travis CI testing
- Replace
bundleDir
byparams.genome_base
- Update
MultiQC
to1.3
(MEGAQC FTW) - Move and rename some test files
- Version of COSMIC GRCh37 v83
- Write an error message when
--sampleDir
does not find any FASTQ files base.config
for ConcatVCF process- File specification for recalibrationReport in RecalibrateBam process (got error on AWS Batch)
- #488 - Better CPU requirements for
ConcatVCF
- #489 - Exception handling for
ASCAT
- #490 - CPU requirements for
runSingleStrelka
andrunSingleManta
- #475 - 16 cpus for local executor
- #357 -
ASCAT
works for GRCh38 - #471 - Running
Singularity
on/scratch
- #480 - No
tsv
file needed for stepannotate
- #479 - Typo in
uppmax-localhost.config
runascat
andrunconvertallelecounts
containers are now replaced byr-base
willmclaren/ensembl-vep:release_90.5
is now base forvepgrch37
andvepgrch38
vep
containerstrelka_config.ini
file
- #471 - Running
Singularity
on /scratch - #472 - Update function to check Nextflow version
- #473 - Remove
returnMin()
function
- Fix version for Manuscript
- Singularity possibilities
- Reports made by default
- Intervals file can be a bed file
- Normal sample preprocessing + HaplotypeCaller is possible
- Better Travis CI tests
- Memory requirements
- Docker possibilities