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Checked in eager/subworkflows/local/utils_nfcore_eager_pipeline/main.nf
No mixed strandedness for the same sample across different libraries is allowed. (eg all data for sampleA must be single stranded, or double stranded library preparations)
Multiple PE libraries must have unique name.
NEW:
Single LibraryID is only allowed to have a single type of UDG treatment
removed filecollision/overwriting of finalbam/raw if raw set as genotyping input (the process gets repeated if run_mapdamage_rescaling, run_pmd_filtering, or run_trim_bam are set), this way keeps only the initial LIBRARY_MERGE subworkflow edition.
Todos:
: decide upon behavior for manipulate damage - currently it can be activated but never used downstream or saved. ideally it automaticaly saves the rescaled/trimmed/maniuplated sample bams post merging. (possibly move merge_library_genotyping to the subworkflow of MANIPULATE_DAMAGE for clarity?) + allow for multiple output saving per tool for this post-damage merging.
check possible genotyping file collision points
generate test dataset with as many weird input combos as possible to find file collisions.
Merges happen at these levels:
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