Alterations to phavu.McClean_Lee_2022.yml on 27 March 2025

Author: ScottKalberer

Phaseolus/vulgaris/studies/phavu.McClean_Lee_2022.yml

@@ -37,6 +37,17 @@ comments:
     functional domains required for P450 activities, evolved independently in
     the two bean gene pools from a common ancestral gene (McClean, Lee et al.,
     2022).
+  - Evidence that V encodes F3′5′H was provided by the discovery of the vlae
+  [M0056] white-flowering haplotype. M0056 was produced by a natural intragenic
+  recombination event between the vlae [MDRK] (pink flower) and v [Mex235]
+  (white flower) haplotypes. A deletion was introduced to the vlae [MDRK]
+  haplotype, resulting in a frame-shift stop codon that eliminated the essential
+  heme-binding and SRS6 regions. This recombination event is a natural
+  equivalent to gene editing in a species, P. vulgaris, in which such
+  complementation technologies have yet to be developed. When a section of the
+  candidate gene is deleted, and the resultant progeny shows a phenotypic
+  change, the candidate gene is revealed as the causative gene for the phenotype
+  (McClean, Lee et al., 2022).
   - Six single-base mutations within the coding region of PvPUR altered the
     protein structure of F3’5’H resulting in the purple phenotype (Liu, Yang et
     al., 2023).