-doc: "Kids First Data Resource Center Alignment and Haplotype Calling Workflow (bam-to-cram-to-gVCF). This pipeline follows Broad best practices outlined in [Data pre-processing for variant discovery.](https://software.broadinstitute.org/gatk/best-practices/workflow?id=11165) Github branch directly related to this app is [here.](https://github.com/kids-first/kf-alignment-workflow/tree/mb-publish-bam-align) It uses bam input and aligns/re-aligns to a bwa-indexed reference fasta, version hg38. Resultant bam is de-dupped and base score recalibrated. Contamination is calculated and a gVCF is created using GATK4 Haplotype caller. Inputs from this can be used later on for further analysis in joint trio genotyping and subsequent refinement and deNovo variant analysis."
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