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workflows/kfdrc_alignment_wf.cwl

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@@ -2,7 +2,7 @@ cwlVersion: v1.0
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class: Workflow
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id: kfdrc-alignment-bam2cram2gvcf
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label: Kids First DRC Alignment Workflow
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doc: "Kids First Data Resource Center Alignment and Haplotype Calling Workflow (bam-to-cram-to-gVCF). This pipeline follows Broad best practices outlined in [Data pre-processing for variant discovery.](https://software.broadinstitute.org/gatk/best-practices/workflow?id=11165) Github branch directly related to this app is [here.](https://github.com/kids-first/kf-alignment-workflow/tree/mb-publish-bam-align) It uses bam input and aligns/re-aligns to a bwa-indexed reference fasta, version hg38. Resultant bam is de-dupped and base score recalibrated. Contamination is calculated and a gVCF is created using GATK4 Haplotype caller. Inputs from this can be used later on for further analysis in joint trio genotyping and subsequent refinement and deNovo variant analysis."
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doc: "Kids First Data Resource Center Alignment and Haplotype Calling Workflow (bam-to-cram-to-gVCF). This pipeline follows Broad best practices outlined in [Data pre-processing for variant discovery.](https://software.broadinstitute.org/gatk/best-practices/workflow?id=11165)\n\n![data service logo](https://github.com/d3b-center/d3b-research-workflows/raw/master/doc/kfdrc-logo-sm.png)\n\nGithub branch directly related to this app is [here.](https://github.com/kids-first/kf-alignment-workflow/tree/mb-publish-bam-align) It uses bam input and aligns/re-aligns to a bwa-indexed reference fasta, version hg38. Resultant bam is de-dupped and base score recalibrated. Contamination is calculated and a gVCF is created using GATK4 Haplotype caller. Inputs from this can be used later on for further analysis in joint trio genotyping and subsequent refinement and deNovo variant analysis."
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sbg:license: "Apache License 2.0"
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sbg:categories: ["Alignment", "DNA", "WGS"]
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requirements:

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