@@ -274,72 +274,71 @@ For the next step we need additional data tracks. Please load `dm3_genes.bed`, `
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> ### {% icon hands_on %} Hands-on: Plotting TADs
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>
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- > 1. **hicPlotTADs ** {% icon tool %}: Run hicPlotTADs adjusting the parameters:
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+ > 1. **pyGenomeTracks ** {% icon tool %}: Run pyGenomeTracks adjusting the parameters:
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> - "Region of the genome to limit the operation" to `chr2L:14500000-16500000`
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> - "Choose style of the track" to `TAD visualization`
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> - "Plot title" to `HiC dm3 chr2L:14500000-16500000`
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> - "Matrix to compute on." to the corrected matrix from hicCorrectMatrix step
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> - "Depth" to `750000`
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- > - "Width " to `4`
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+ > - "Height " to `4`
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> - "Boundaries file" to `hicFindTads on data XX: TAD domains`
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> - "Show x labels" to `Yes`
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>
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> - "+Insert Include tracks in your plot"
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> - "Choose style of the track" to `Bedgraph matrix track`
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> - "Plot title" to `TAD separation score`
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> - "Track file bedgraph format" to `hicFindTads on data XX: TAD information in bm file`
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- > - "Width " to `4`
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+ > - "Height " to `4`
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> - "Set type to lines" to 'True'
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>
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>
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> - "+Insert Include tracks in your plot"
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> - "Choose style of the track" to `Bigwig track`
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> - "Plot title" to `PC1`
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> - "Track file bigwig format" the first computed `hicPCA` result
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- > - "Minimum value" to `-0.03`
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- > - "Maximum value" to `0.03`
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- > - "Width" to `1.5`
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+ > - "Height" to `1.5`
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> - "Color of track" to a color of your choice
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>
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> - "+Insert Include tracks in your plot"
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> - "Choose style of the track" to `Bigwig track`
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> - "Plot title" to `PC2`
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> - "Track file bigwig format" the second computed `hicPCA` result
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- > - "Minimum value" to `-0.03`
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- > - "Maximum value" to `0.03`
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- > - "Width" to `1.5`
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+ > - "Height" to `1.5`
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> - "Color of track" to a color of your choice
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>
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> - "+Insert Include tracks in your plot"
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> - "Choose style of the track" to `Bigwig track`
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> - "Plot title" to `H3K36me3`
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> - "Track file bigwig format" to `H3K36me3`
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- > - "Width " to `1.5`
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+ > - "Height " to `1.5`
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> - "Color of track" to a color of your choice
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>
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> - "+Insert Include tracks in your plot"
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> - "Choose style of the track" to `Bigwig track`
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> - "Plot title" to `H3K27me3`
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> - "Track file bigwig format" to `H3K27me3`
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- > - "Width " to `1.5`
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+ > - "Height " to `1.5`
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> - "Color of track" to a color of your choice
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>
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> - "+Insert Include tracks in your plot"
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> - "Choose style of the track" to `Bigwig track`
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> - "Plot title" to `H4K16ac`
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> - "Track file bigwig format" to `H4K16ac`
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- > - "Width " to `1.5`
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+ > - "Height " to `1.5`
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> - "Color of track" to a color of your choice
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>
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> - "+Insert Include tracks in your plot"
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> - "Choose style of the track" to `Gene track`
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> - "Plot title" to `dm3 genes`
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> - "Track file bedgraph format" the imported .bed file
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- > - "Width " to `3`
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+ > - "Height " to `3`
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> - "Type" to `genes`
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> - "Gene rows" to `15`
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> - "Color of track" to a color of your choice
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>
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+ > - "+Insert Include tracks in your plot"
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+ > - "Choose style of the track" to `Vlines track`
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+ > - "Track file bed format" to `hicFindTads on data XX: TAD domains`
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{: .hands_on}
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The resulting image should look like this one:
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