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| 1 | +#!/usr/bin/env cwl-runner |
| 2 | +# |
| 3 | +# Authors: [email protected] and [email protected] UNIVERSITY OF MELBOURNE |
| 4 | +# Developed for CWL consortium http://commonwl.org/ |
| 5 | + |
| 6 | +#!/usr/bin/env cwl-runner |
| 7 | +class: CommandLineTool |
| 8 | + |
| 9 | +description: | |
| 10 | + Generate base recalibration table to compensate for systematic errors in basecalling confidences |
| 11 | + Options: |
| 12 | + -T BaseRecalibrator Tool name to be executed |
| 13 | + -R reference.fasta Reference sequence in fasta format |
| 14 | + -I my_reads.bam bam file produced from indelRealigner |
| 15 | + -knownSites latest_dbsnp.vcf set of known indels |
| 16 | + -o recal_data.table Output file name |
| 17 | + |
| 18 | +requirements: |
| 19 | + - import: node-engine.cwl |
| 20 | + - import: envvar-global.cwl |
| 21 | + - import: gatk-docker.cwl |
| 22 | + |
| 23 | +arguments: |
| 24 | + - valueFrom: "/home/biodocker/bin/gatk/target/GenomeAnalysisTK.jar" |
| 25 | + position: 2 |
| 26 | + prefix: "-jar" |
| 27 | + |
| 28 | +inputs: |
| 29 | + |
| 30 | + - id: "#java_arg" |
| 31 | + type: string |
| 32 | + default: "-Xmx4g" |
| 33 | + inputBinding: |
| 34 | + position: 1 |
| 35 | + |
| 36 | + - id: "#BaseRecalibrator" |
| 37 | + type: string |
| 38 | + default: "BaseRecalibrator" |
| 39 | + inputBinding: { position: 3, prefix: "-T" } |
| 40 | + description: tool used for this step from GATK jar |
| 41 | + |
| 42 | + - id: "#reference" |
| 43 | + type: File |
| 44 | + inputBinding: |
| 45 | + position: 4 |
| 46 | + prefix: "-R" |
| 47 | + secondaryFiles: |
| 48 | + - ".amb" |
| 49 | + - ".ann" |
| 50 | + - ".bwt" |
| 51 | + - ".pac" |
| 52 | + - ".rbwt" |
| 53 | + - ".rpac" |
| 54 | + - ".rsa" |
| 55 | + - ".sa" |
| 56 | + - ".fai" |
| 57 | + - "^.dict" |
| 58 | + |
| 59 | + - id: "#inputBam_BaseRecalibrator" |
| 60 | + type: File |
| 61 | + description: bam file produced after indelRealigner |
| 62 | + inputBinding: |
| 63 | + position: 5 |
| 64 | + prefix: "-I" |
| 65 | + secondaryFiles: |
| 66 | + - "^.bai" |
| 67 | + |
| 68 | + - id: "#known" |
| 69 | + type: |
| 70 | + type: array |
| 71 | + items: File |
| 72 | + inputBinding: { prefix: "-knownSites" } |
| 73 | + inputBinding: |
| 74 | + position: 6 |
| 75 | + |
| 76 | + - id: "#outputfile_BaseRecalibrator" |
| 77 | + type: string |
| 78 | + description: name of the output file from baseRecalibrator |
| 79 | + inputBinding: |
| 80 | + position: 7 |
| 81 | + prefix: "-o" |
| 82 | + |
| 83 | +outputs: |
| 84 | + - id: "#output_baseRecalibrator" |
| 85 | + type: File |
| 86 | + outputBinding: |
| 87 | + glob: |
| 88 | + engine: cwl:JsonPointer |
| 89 | + script: /job/outputfile_BaseRecalibrator |
| 90 | + |
| 91 | +baseCommand: ["java"] |
| 92 | + |
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