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config.yaml
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samples: list.txt
adapters:
SE: "adapters_SE.fa"
flexbar:
adapter-trim-end: "RIGHT"
adapter-min-overlap: 7
pre-trim-left: 5
max-uncalled: 150
min-read-length: 25
qtrim: "TAIL"
qtrim-format: "sanger"
qtrim-threshold: 20
zip-output: "GZ"
bt2idx: "refs/hg19/Sequence/Bowtie2Index/genome"
bowtie2:
sensitive: ""
rg: "PL:IonTorrent"
mh_bedfile: "refs/chr1_mh.bed"
fasta_ref: "refs/hg19/Sequence/Bowtie2Index/genome.fa"
freebayes:
variant:
min-alternate-count: "5"
min-mapping-quality: "20"
min-base-quality: "30"
use-duplicate-reads: ""
haps:
min-mapping-quality: "20"
min-base-quality: "30"
use-duplicate-reads: ""
min-repeat-size: "0"
no-partial-observations: ""
max-complex-gap: "400"
haplotype-length: "500"
vcffilter_qual: 20