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:alt:An expanded mouse lung imaged with Axially Swept Light-Sheet Microscopy
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The Dean Lab is a multi-disciplinary team of biologists, chemists, physicists, and engineers
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at the University of Texas Southwestern Medical Center in Dallas, Texas. We develop
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of their native environment, and how these decisions go awry in disease. More information
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about our research can be found on our `website <https://www.dean-lab.org>`_.
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**Projects**
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=============
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---------------
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**Research Projects**
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=====================
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- Light-sheet microscopy software packages for intelligent imaging of complex tissue samples
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- `navigate <https://thedeanlab.github.io/navigate/>`_ - Light-sheet microscopy software packages for intelligent imaging of complex tissue samples. navigate is equipped with a series of plugins that extend its functionality, enabling the use of `robotics <https://github.com/TheDeanLab/navigate-at-scale>`_, imaging cells in a `projection format <https://github.com/TheDeanLab/navigate-confocal-projection>`_, accessing additional `hardware <https://github.com/TheDeanLab/navigate-mmcore-plugin>`_, and leveraging real-time feedback from `ilastik <https://github.com/TheDeanLab/navigate-ilastik-server>`_. To enable users to generate their own plugins, we provide a `plugin template <https://github.com/TheDeanLab/navigate-plugin-template>`_.
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- Tissue clearing and labeling protocols for high-resolution imaging deep within
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biological specimens.
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- `Altair <https://thedeanlab.github.io/altair/>`_ - A series of cost-effective, easy-to-assemble, and performant light-sheet microscopes for interrogating cells and tissues at the highest resolution.
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---------------
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- Cost-effective, easy-to-assemble, and performant light-sheet microscopes for interrogating live cells,
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expanded, and cleared tissue samples.
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**Educational Resources**
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================
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=========================
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- `CI2023 <https://github.com/TheDeanLab/CI2023>`_ - An introduction to continuous integration and continuous deployment for scientific software.
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---------------
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**Publication Repositories**
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================
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============================
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For many manuscripts published over the years, we provide access to CAD files, software, and data
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via GitHub. These repositories are organized by publication year.
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- `2015, Dean <https://github.com/AdvancedImagingUTSW/manuscripts/tree/main/2015-dean>`_ - Deconvolution-free subcellular imaging with axially swept light sheet microscopy.
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- `2016, Dean <https://github.com/AdvancedImagingUTSW/manuscripts/tree/main/2016-dean>`_ - Diagonally scanned light-sheet microscopy for fast volumetric imaging of adherent cells.
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- `2016, Welf <https://github.com/AdvancedImagingUTSW/manuscripts/tree/main/2016-welf-meSPIM>`_ - Quantitative multiscale cell imaging in controlled 3D microenvironments.
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- `2019, Chakraborty <https://github.com/AdvancedImagingUTSW/manuscripts/tree/main/2019-chakraborty>`_ - Light-sheet microscopy of cleared tissues with isotropic, subcellular resolution.
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- `2020, Chang <https://github.com/AdvancedImagingUTSW/manuscripts/tree/main/2020-chang-systematic-comparison>`_ - Systematic and quantitative comparison of lattice and Gaussian light-sheets.
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- `2020, Sapoznik <https://github.com/AdvancedImagingUTSW/manuscripts/tree/main/2020-sapoznik-oblique-plane-microscopy>`_ - A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics.
- `2022, Dean <https://github.com/AdvancedImagingUTSW/manuscripts/tree/main/2021-dean-protocol>`_ - Isotropic imaging across spatial scales with axially swept light-sheet microscopy.
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- `2022, Chang <https://github.com/AdvancedImagingUTSW/manuscripts/tree/main/2022-chen>`_ - Resolution doubling in light-sheet microscopy via oblique plane structured illumination.
- `TBD, Isogai <https://github.com/AdvancedImagingUTSW/manuscripts/tree/main/2022-isogai>`_ - Direct Arp2/3-vinculin binding is essential for cell spreading, but only on compliant substrates and in 3D.
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**Funding**
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============
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via GitHub. These repositories are organized by publication year. You can find this information :ref:`publications-home`.
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---------------
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**Our Supporters**
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==================
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The **Dean Lab** is supported by the following funding sources:
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@@ -61,12 +51,9 @@ The **Dean Lab** is supported by the following funding sources:
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- `UTSW President's Research Council <https://engage.utsouthwestern.edu/pages/membership-giving/membership-giving---presidents-research-council>`_
A list of our publications with links to the associated GitHub repositories. These repositories
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contain the source code, data, and CAD files associated with the publication. A comprehensive list of our publications can be found on our website at `www.dean-lab.org <www.dean-lab.org>`_, `Google Scholar <https://scholar.google.com/citations?user=Uv0B5xIAAAAJ&hl>`_, or `PubMed <https://www.ncbi.nlm.nih.gov/myncbi/kevin.dean.1/bibliography/public/>`_.
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- `2015, Dean <https://github.com/AdvancedImagingUTSW/manuscripts/tree/main/2015-dean>`_ - Deconvolution-free subcellular imaging with axially swept light sheet microscopy.
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- `2016, Dean <https://github.com/AdvancedImagingUTSW/manuscripts/tree/main/2016-dean>`_ - Diagonally scanned light-sheet microscopy for fast volumetric imaging of adherent cells.
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- `2016, Welf <https://github.com/AdvancedImagingUTSW/manuscripts/tree/main/2016-welf-meSPIM>`_ - Quantitative multiscale cell imaging in controlled 3D microenvironments.
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- `2019, Chakraborty <https://github.com/AdvancedImagingUTSW/manuscripts/tree/main/2019-chakraborty>`_ - Light-sheet microscopy of cleared tissues with isotropic, subcellular resolution.
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- `2020, Chang <https://github.com/AdvancedImagingUTSW/manuscripts/tree/main/2020-chang-systematic-comparison>`_ - Systematic and quantitative comparison of lattice and Gaussian light-sheets.
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- `2020, Sapoznik <https://github.com/AdvancedImagingUTSW/manuscripts/tree/main/2020-sapoznik-oblique-plane-microscopy>`_ - A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics.
- `2022, Dean <https://github.com/AdvancedImagingUTSW/manuscripts/tree/main/2021-dean-protocol>`_ - Isotropic imaging across spatial scales with axially swept light-sheet microscopy.
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- `2022, Chang <https://github.com/AdvancedImagingUTSW/manuscripts/tree/main/2022-chen>`_ - Resolution doubling in light-sheet microscopy via oblique plane structured illumination.
- `TBD, Isogai <https://github.com/AdvancedImagingUTSW/manuscripts/tree/main/2022-isogai>`_ - Direct Arp2/3-vinculin binding is essential for cell spreading, but only on compliant substrates and in 3D.
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