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| 1 | +# This is a configuration file for fastq_screen |
| 2 | + |
| 3 | +############ |
| 4 | +## Bowtie2 # |
| 5 | +############ |
| 6 | +## If the Bowtie2 binary is not in your PATH then you can |
| 7 | +## set this value to tell the program where to find it. |
| 8 | +## Uncomment the line below and set the appropriate location |
| 9 | + |
| 10 | +#BOWTIE2 /bi/apps/bowtie2/2.3.2/bowtie2 |
| 11 | + |
| 12 | + |
| 13 | +############ |
| 14 | +## BISMARK # |
| 15 | +############ |
| 16 | +## If the Bismark binary is not in your PATH then you can |
| 17 | +## set this value to tell the program where to find it. |
| 18 | +## Uncomment the line below and set the appropriate location |
| 19 | + |
| 20 | +#BISMARK /bi/apps/bismark/bismark |
| 21 | + |
| 22 | + |
| 23 | +############ |
| 24 | +## Threads # |
| 25 | +############ |
| 26 | +## Bowtie can be made to run across multiple CPU cores to |
| 27 | +## speed up your searches. Set this value to the number |
| 28 | +## of cores you want to use for your searches. |
| 29 | + |
| 30 | +THREADS 7 |
| 31 | + |
| 32 | +############## |
| 33 | +## Databases # |
| 34 | +############## |
| 35 | +## This section allows you to configure multiple databases |
| 36 | +## to search against in your screen. For each database |
| 37 | +## you need to provide a database name (which can't contain |
| 38 | +## spaces) and the location of the bowtie indices which |
| 39 | +## you created for that database. |
| 40 | +## |
| 41 | +## The default entries shown below are only suggested examples |
| 42 | +## you can add as many DATABASE sections as you like, and you |
| 43 | +## can comment out or remove as many of the existing entries |
| 44 | +## as you like. |
| 45 | + |
| 46 | + |
| 47 | + |
| 48 | +######### |
| 49 | +## Human - sequences available from |
| 50 | +## ftp://ftp.ensembl.org/pub/current/fasta/homo_sapiens/dna/ |
| 51 | +DATABASE Human [FastQ_Screen_Genomes_Path]/Human/GRCh38 |
| 52 | + |
| 53 | + |
| 54 | + |
| 55 | +######### |
| 56 | +## Mouse - sequence available from |
| 57 | +## ftp://ftp.ensembl.org/pub/current/fasta/mus_musculus/dna/ |
| 58 | +DATABASE Mouse [FastQ_Screen_Genomes_Path]/Mouse/GRCm38 |
| 59 | + |
| 60 | + |
| 61 | + |
| 62 | +######### |
| 63 | +## Rat - sequence available from |
| 64 | +## ftp://ftp.ensembl.org/pub/current/fasta/rattus_norvegicus/dna/ |
| 65 | +DATABASE Rat [FastQ_Screen_Genomes_Path]/Rat/Rnor_6.0 |
| 66 | + |
| 67 | + |
| 68 | + |
| 69 | +############ |
| 70 | +# Drosophila |
| 71 | +DATABASE Drosophila [FastQ_Screen_Genomes_Path]/Drosophila_melanogaster/BDGP6 |
| 72 | + |
| 73 | + |
| 74 | + |
| 75 | +######### |
| 76 | +## Worm |
| 77 | +DATABASE Worm [FastQ_Screen_Genomes_Path]/C_elegans/WBcel235 |
| 78 | + |
| 79 | + |
| 80 | + |
| 81 | +######### |
| 82 | +## Yeast - sequence available from |
| 83 | +## ftp://ftp.ensembl.org/pub/current/fasta/saccharomyces_cerevisiae/dna/ |
| 84 | +DATABASE Yeast [FastQ_Screen_Genomes_Path]/Yeast/R64-1-1 |
| 85 | + |
| 86 | + |
| 87 | + |
| 88 | +######### |
| 89 | +## Arabidopsis - sequences available from |
| 90 | +DATABASE Arabidopsis [FastQ_Screen_Genomes_Path]/Arabidopsis/TAIR10 |
| 91 | + |
| 92 | + |
| 93 | + |
| 94 | +######### |
| 95 | +## Ecoli |
| 96 | +## Sequence available from EMBL accession U00096.2 |
| 97 | +DATABASE Ecoli [FastQ_Screen_Genomes_Path]/E_coli/NC_010473 |
| 98 | + |
| 99 | + |
| 100 | + |
| 101 | + |
| 102 | +######## |
| 103 | +## PhiX - sequence available from Refseq accession NC_001422.1 |
| 104 | +DATABASE PhiX [FastQ_Screen_Genomes_Path]/PhiX/phiX174_plus_SNPs |
| 105 | + |
| 106 | + |
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