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I'm using Clampfit v11.2 template matching method to detect synaptic events in a gap-free recording acquired using Clampex v11.2. I am then importing the abf file created from all the detected events into Igor using NeuroMatic v3.0s for further analysis.
When I import the abf file generated during event detection, the imported wave(s) doesn't seem to have correct Y scaling for some reason.
Here is an example:
Clampfit file
Igor Pro wave
The Y scaling is incorrect in the Igor Wave. The Y scaling of the abf file is fine when I reopen it in Clampfit. I'm guessing it might have something to do with the creation of the abf file in Clampfit, because the scaling of the original gap-free recording (created from Clampex) is fine when imported into Igor. Do you know if there is a different scaling factor hidden somewhere? It doesn't seem to be related to the 'Scale' in the Wave note.
Hi,
I'm using Clampfit v11.2 template matching method to detect synaptic events in a gap-free recording acquired using Clampex v11.2. I am then importing the abf file created from all the detected events into Igor using NeuroMatic v3.0s for further analysis.
When I import the abf file generated during event detection, the imported wave(s) doesn't seem to have correct Y scaling for some reason.
Here is an example:
Clampfit file
![clampfit](https://private-user-images.githubusercontent.com/18335331/309903276-b37ad1f8-16f4-48a6-926a-3c76971c0c9e.PNG?jwt=eyJhbGciOiJIUzI1NiIsInR5cCI6IkpXVCJ9.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.pAs7bmVonGkVPWWiWus0sfsBJreqenzkS-UN_4ICB1M)
Igor Pro wave
![igor_import](https://private-user-images.githubusercontent.com/18335331/309903287-3b8a6b25-8a67-4cfe-9f47-e2d74ce55f72.PNG?jwt=eyJhbGciOiJIUzI1NiIsInR5cCI6IkpXVCJ9.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.g_ITrOZMGJkEyD45fOs1GbK9jVL3Dzi2OCNmbz5A53A)
The Y scaling is incorrect in the Igor Wave. The Y scaling of the abf file is fine when I reopen it in Clampfit. I'm guessing it might have something to do with the creation of the abf file in Clampfit, because the scaling of the original gap-free recording (created from Clampex) is fine when imported into Igor. Do you know if there is a different scaling factor hidden somewhere? It doesn't seem to be related to the 'Scale' in the Wave note.
It seems to be fine using Clampex/Clampfit 10.7.
An example of the abf file can be found here
Thanks so much!
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