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Importing abf files generated using Clampfit 11.2 Event Detection function #21

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damianjwilliams opened this issue Mar 4, 2024 · 0 comments

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@damianjwilliams
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damianjwilliams commented Mar 4, 2024

Hi,

I'm using Clampfit v11.2 template matching method to detect synaptic events in a gap-free recording acquired using Clampex v11.2. I am then importing the abf file created from all the detected events into Igor using NeuroMatic v3.0s for further analysis.

When I import the abf file generated during event detection, the imported wave(s) doesn't seem to have correct Y scaling for some reason.

Here is an example:

Clampfit file
clampfit

Igor Pro wave
igor_import

The Y scaling is incorrect in the Igor Wave. The Y scaling of the abf file is fine when I reopen it in Clampfit. I'm guessing it might have something to do with the creation of the abf file in Clampfit, because the scaling of the original gap-free recording (created from Clampex) is fine when imported into Igor. Do you know if there is a different scaling factor hidden somewhere? It doesn't seem to be related to the 'Scale' in the Wave note.

It seems to be fine using Clampex/Clampfit 10.7.

An example of the abf file can be found here

Thanks so much!

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