@@ -7,22 +7,22 @@ mapped HiFi reads.
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To use ` aligned_bam_to_cpg_scores ` download the latest release tarball compiled for 64-bit linux platforms on the
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[ github release channel] ( https://github.com/PacificBiosciences/pb-CpG-tools/releases/latest ) , then unpack the tar file.
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- As an example, the v2.1.1 release can be obtained as follows:
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+ As an example, the v2.2.0 release can be obtained as follows:
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- wget https://github.com/PacificBiosciences/pb-CpG-tools/releases/download/v2.1.1 /pb-CpG-tools-v2.1.1 -x86_64-unknown-linux-gnu.tar.gz
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- tar -xzf pb-CpG-tools-v2.1.1 -x86_64-unknown-linux-gnu.tar.gz
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+ wget https://github.com/PacificBiosciences/pb-CpG-tools/releases/download/v2.2.0 /pb-CpG-tools-v2.2.0 -x86_64-unknown-linux-gnu.tar.gz
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+ tar -xzf pb-CpG-tools-v2.2.0 -x86_64-unknown-linux-gnu.tar.gz
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# Run help option to test binary and see latest usage details:
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- pb-CpG-tools-v2.1.1 -x86_64-unknown-linux-gnu/bin/aligned_bam_to_cpg_scores --help
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+ pb-CpG-tools-v2.2.0 -x86_64-unknown-linux-gnu/bin/aligned_bam_to_cpg_scores --help
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` aligned_bam_to_cpg_scores ` includes a number of ways to summarize site propabilities for 5mC methylation,
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detailed below. The recommended default workflow will use the ` model ` pileup mode and ` denovo ` modsites mode.
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Continuing from the example above, the script below runs the tool on a mapped WGS bam for HG002:
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- pb-CpG-tools-v2.1.1 -x86_64-unknown-linux-gnu/bin/aligned_bam_to_cpg_scores \
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+ pb-CpG-tools-v2.2.0 -x86_64-unknown-linux-gnu/bin/aligned_bam_to_cpg_scores \
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--bam HG002.hg38.pbmm2.bam \
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--output-prefix HG002.hg38.pbmm2 \
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- --model pb-CpG-tools-v2.1.1 -x86_64-unknown-linux-gnu/models/pileup_calling_model.v1.tflite \
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+ --model pb-CpG-tools-v2.2.0 -x86_64-unknown-linux-gnu/models/pileup_calling_model.v1.tflite \
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--threads 8
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See the cmdline usage help for the full list of other commandline options:
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