Analyze_Calcium_Signals_In_Spines

Analyze calcium signals in dendritic spines. The images consist of time-series of calcium signals. Each image contains a selection that marks the point of stimulation. The tool finds the region to analyze close to the point of stimulation. It measures the intensity of the calcium signal in the whole region of interest and in the segmented spots.

The source code in git-hub can be found here.

Getting started

To install the tool save the file analyze_calcium_signals_in_dendritic_spines.ijm into the folder macros/toolsets of your FIJI installation and restart FIJI.

Select the "analyze_calcium_signals_in_dendritic_spines" toolset from the >> button of the ImageJ launcher.

• The first button (the one with the image) opens this help page
• The f-button finds the region to analyze near the stimulation point on the current image. The first roi in the roi-manager must be the one marking the stimulation point.
• The a-button runs the whole analysis of the calcium signal on the current image.
• The b-button runs the analysis of the calcium signal in batch-mode on a set of images in a folder.
• The r-button runs the region detection in batch-mode on a set of images in a folder.