From 996a1f2a64bb189d10b305015a33cd6c3d554681 Mon Sep 17 00:00:00 2001 From: Alicia Schep Date: Tue, 21 Apr 2015 11:58:39 -0700 Subject: [PATCH] updated samtools link in docs --- docs/index.md | 2 +- docs/nucleoatac.md | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/docs/index.md b/docs/index.md index b0699e7..657aab2 100644 --- a/docs/index.md +++ b/docs/index.md @@ -10,7 +10,7 @@ NucleoATAC is a python package for calling nucleosome positions and occupancy us ###Needed files * Aligned paired-end reads in BAM format. Must be sorted & indexed. Probably should be filtered for quality. -* Fasta file with genome reference. Must be indexed by faidx from [samtools](http://samtools.sourceforge.net/). +* Fasta file with genome reference. Must be indexed by faidx from [samtools](http://www.htslib.org/). * Bed file with regions to perform analysis. Generally will be broad open chromatin regions. ###Installation diff --git a/docs/nucleoatac.md b/docs/nucleoatac.md index c4c95ab..81c5fd2 100644 --- a/docs/nucleoatac.md +++ b/docs/nucleoatac.md @@ -5,7 +5,7 @@ Prior to using nucleoatac to call nucleosomes, you will need at least three type 1) Bam file with aligned reads. These generally will be filtered for reads not mapping to mitochondria & reads with high mapping quality. -2) Fasta file with genome used in alignment. This file must be indexed (using [samtools](http://samtools.sourceforge.net/samtools.shtml#2) faidx) +2) Fasta file with genome used in alignment. This file must be indexed (using [samtools](http://www.htslib.org/) faidx) 3) Sorted bed file with regions for which nucleosome analysis is to be performed. These regions will generally be broad open-chromatin regions (i.e. regions called by MACS2 with the --broad flag). It is potentially advisable to extend these regions a bit (e.g. using bedtools slop). Regions should not overlap so it is advisable to use bedtools merge on these regions.