When I ran CAT, the following error occurred. The following is the input file and code I provided. I don't know what caused this error.
I only provide hal file and second generation transcriptome data here, gff is only the result of one chromosome, and transcriptome data is the transcriptome data of all autosomes.I hope someone will help me.
[ANNOTATION] ZS11 = test_data/ZS11.v0.A01.change.gff3 [BAM] ZS11 = /home/jwli/data4/prediction/CAT/Comparative-Annotation-Toolkit/test_data/bams/ZS11/252_20DAF.srt.bam,/home/jwli/data4/prediction/CAT/Comparative-Annotation-Toolkit/test_data/bams/ZS11/454_40DAF.srt.bam,/home/jwli/data4/prediction/CAT/Comparative-Annotation-Toolkit/test_data/bams/ZS11/B4_3.srt.bam,/home/jwli/data4/prediction/CAT/Comparative-Annotation-Toolkit/test_data/bams/ZS11/SRR6453768.srt.bam,/home/jwli/data4/prediction/CAT/Comparative-Annotation-Toolkit/test_data/bams/ZS11/SRR6453769.srt.bam
luigi --module cat RunCat --hal=test_data/Brassica_napus15-pg.full.hal --ref-genome=Darmorv4.1 --workers=10 --config=test_data/Darmor_A01.config --work-dir Darmor_A01 --out-dir Darmor_A01 --local-scheduler --augustus --augustus-species=arabidopsis --augustus-cgp --binary-mode local --assembly-hub > log_darmor_a01.txt